FBXO4 antibody (AA 250-262)

Catalog No. ABIN962656

Product Details anti-FBXO4 Antibody

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Binding Specificity: AA 250-262

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FBXO4 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human FBXO4. This antibody is expected to recognize isoform 1 and 2 (NP_036308.1, NP_277019.1).

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla (100%) Gibbon (92%) Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide C-EHTSAVNKMFSRH from an internal region of human FBXO4 (NP_036308.1, NP_277019.1). Percent identity by BLAST analysis: Human, Gorilla (100%), Gibbon (92%), Monkey, Marmoset, Elephant, Panda, Dog, Bat, Bovine, Rabbit, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 - 3.75 μg/mL), Peptide-ELISA (1:8000)

Usage: Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Preliminary experiments gave an approx 48  kDa band in Human Heart ysates after 1 μg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.1  kDa according to NP_036308.1. The 48  kDa band was successfully blocked by incubation with the immunizing peptide.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Tris-buffered saline,  pH 7.3, 0.5 % BSA, 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FBXO4

Target: FBXO4 (F-Box Protein 4 (FBXO4))

Alternative Name: FBXO4 / FBX4 (FBXO4 Products)

Synonyms: FBX4 antibody, 1700096C12Rik antibody, AI851261 antibody, AW494535 antibody, Fbx4 antibody, FBXO4 antibody, fbx4 antibody, si:dkey-46a10.2 antibody, F-box protein 4 antibody, F-box protein 4 L homeolog antibody, FBXO4 antibody, Fbxo4 antibody, fbxo4 antibody, fbxo4.L antibody

Background: Name/Gene ID: FBXO4
Family: F-Box

Synonyms: FBXO4, F-box protein 4, F-box protein Fbx4, FBX4, F-box only protein 4

Gene ID: 26272

NCBI Accession: NP_036308, NP_277019

UniProt: Q9UKT5