Rabbit anti-Human IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN964848

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.

Characteristics: F(ab')2 Human IgG detects human IgG heavy and light chains. F(ab')2 Anti-Human IgG antibody is ideal for investigators invovled in serum protein component research.

Purification: F(ab')2 Anti-Human IgG antibody is a F(ab')2 fragment of IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and pepsin digestion followed by chromatographic separation and extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Human IgG and Human Serum.  No reaction was observed against anti-Rabbit IgG F(c) or anti-Pepsin.

Immunogen:

Immunogen: Human IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 Anti-Human IgG antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

ELISA Dilution: 1:20,000 - 1:100,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 2.0 mL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 10.0 mg/mL

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Centrifuge product if not completely clear after standing at room temperature.
Dilute only prior to immediate use.

Storage: RT,4 °C,-20 °C

Storage Comment: This product is stable for several weeks at 4 °C as an undiluted liquid. Aliquot contents and freeze at -24 °C or below for extended storage.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: rabbit F(ab')2 anti-Human IgG Antibody, rabbit Fab2 anti-Human IgG antibody

Background: F(ab')2 Anti-Human IgG (H&L) Antibody generated in rabbit detects immunoglobulin g from human, both heavy and light chains of the antibody molecule are present. Each IgG has two antigen binding sites. Representing approximately 75 % of serum immunoglobulins in humans, IgG is the most abundant antibody isotype found in the circulation. IgG molecules are synthesized and secreted by plasma B cells. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.