PRKAA1 antibody

Catalog No. ABIN965548

This Rabbit Polyclonal antibody specifically detects PRKAA1 in IHC. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for PRKAA1 antibody (ABIN965548)

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-PRKAA1 Antibody

Specificity: AMPK1 (Ab-174) antibody detects endogenous levels of total AMPK1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1 around the phosphorylation site of threonine 174 (L-R-TP-S-C).

Application Details

Application Notes: IHC: 1:50-1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-PRKAA1 antibody (ABIN965548)

Hurley, Anderson, Franzone, Kemp, Means, Witters: "The Ca2+/calmodulin-dependent protein kinase kinases are AMP-activated protein kinase kinases." in: The Journal of biological chemistry, Vol. 280, Issue 32, pp. 29060-6, (2005) (PubMed).

Woods, Johnstone, Dickerson, Leiper, Fryer, Neumann, Schlattner, Wallimann, Carlson, Carling: "LKB1 is the upstream kinase in the AMP-activated protein kinase cascade." in: Current biology : CB, Vol. 13, Issue 22, pp. 2004-8, (2003) (PubMed).

da Silva Xavier, Leclerc, Salt, Doiron, Hardie, Kahn, Rutter: "Role of AMP-activated protein kinase in the regulation by glucose of islet beta cell gene expression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 97, Issue 8, pp. 4023-8, (2000) (PubMed).

Target Details for PRKAA1

Target: PRKAA1 (Protein Kinase, AMP-Activated, alpha 1 Catalytic Subunit (PRKAA1))

Alternative Name: AMPK1

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Warburg Effect