Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2) antibody
| Antigen |
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| Synonyms |
AMPK, AMPK2, PRKAA, A830082D05, AMPKalpha2, 2310008I11Rik, AMPKA2, prkaa1, PRKAA2, DKFZp468F2023 |
| Clonality |
Polyclonal |
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Host
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Reactivity
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Conjugate
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Application
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Western Blotting (WB) (55), ELISA (44), Immunofluorescence (IF) (34), Immunohistochemistry (IHC) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (6), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1)
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| Catalog no. |
ABIN965550 |
| Quantity |
100ug (100ug/100ul.) (Variants) |
| Price |
385.00 $ Plus shipping costs $45.00
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Bulk discount
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| Shipping to |
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| Availability |
Will be delivered in 5 to 7 Business Days |
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Alternative name
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AMPK1/AMPK2
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Immunogen
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The antiserum was produced against synthesized non-phosphopeptide derived from human AMPK1/AMPK2 around the phosphorylation site of serine 485 or serine 491 (S-G-SP-V-S).
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Specificity
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AMPK1/AMPK2 (Ab-485/491) antibody detects endogenous levels of total AMPK1/ AMPK2 protein.
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Application Notes
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IHC: 1:50-1:100.
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Concentration
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100ug/100ul.
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Purification
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The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Buffer
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Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
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Preservative
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0.02% Sodium Azide
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Storage
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Store at -20°C/1 year.
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Restrictions
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For Research Use only
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Product
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Woods, Vertommen, Neumann et al.: "Identification of phosphorylation sites in AMP-activated protein kinase (AMPK) for upstream AMPK kinases and study of their roles by site-directed mutagenesis." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28434-42, 2003 (PubMed).
Kim, Tannenbaum, White: "Global phosphoproteome of HT-29 human colon adenocarcinoma cells." in: Journal of proteome research, Vol. 4, Issue 4, pp. 1339-46, 2005 (PubMed).
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Alternatives for antigen "Protein Kinase, AMP-Activated, alpha 2 Catalytic Subunit (PRKAA2)", type "Antibodies"
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Hosts
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Rabbit (85), Mouse (4), Goat (3)
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Reactivities
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Human (87), Mouse (Murine) (51), Rat (Rattus) (49), Cow (Bovine) (38), Pig (Porcine) (37), Dog (Canine) (19), Horse (Equine) (18), Sheep (Ovine) (18)
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Applications
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Western Blotting (WB) (55), ELISA (44), Immunofluorescence (IF) (34), Immunohistochemistry (IHC) (15), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunoprecipitation (IP) (6), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Dot Blot (Dot) (1), Enzyme Immunoassay (EIA) (1), Flow Cytometry (FACS) (1), Immunocytochemistry (ICC) (1)
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Conjugates
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Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy5.5 (2), PE,Cy7 (2)
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Epitopes
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C-Term (27), pSer173 (18), Internal Region (4), Center (2), Center,pThr172 (2), pSer485, pSer491 (2), AA 152-181 (1), AA 353-367 (1), Internal Region,pThr172,AA 148-177 (1), N-Term (1), pSer175 (1), pSer485,pSer491 (1), pThr172 (1)
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Alternatives 
