Apolipoprotein A-V (APOA5) antibody

Antigen: Apolipoprotein A-V (APOA5)

Synonyms: RAP3, Apoav, 1300007O05Rik, APOAV, APOA-V, MGC126836, MGC126838, MGC108612, APOA5, MGC140487, rap3, apoav, apoa-v

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN965569

Additional Information

Alternative name: APOA5

Gene ID: 116519

Isotype: IgG1

Description: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor- agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Despite these compelling molecular biology data, relatively little is known about ApoA5 protein in human serum. This antibody pair detected recombinant apoa5 protein in sandwich ELISA format and could be potential reagents for the development of clinical diagnostic kits.

Specificity: Ni-NTA purified recombinant human Apoa5 expressed in E. Coli strain BL21 (DE3).

Application Details

Application Notes: Western Blot: Dilution 1: 1,000- 1: 2,000
Suggested ELISA dilution 1: 4,000
ptimized dilution must be determined by end user.

Purification: Antibodies are purified by protein G affinity chromatography.

Storage: Store at -20° C.

Restrictions: For Research Use only

Publications

Product: Pennacchio, Olivier, Hubacek et al.: "An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing." in: Science (New York, N.Y.), Vol. 294, Issue 5540, pp. 169-73, 2001 (PubMed).

Prieur, Coste, Rodriguez: "The human apolipoprotein AV gene is regulated by peroxisome proliferator-activated receptor-alpha and contains a novel farnesoid X-activated receptor response element." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25468-80, 2003 (PubMed).