Apoa5(ab) antibody

Antigen: Apoa5(ab)

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN965571

Additional Information

Gene ID: 116519

Isotype: IgG1

Description: Apolipoprotein A5 (ApoA5) is fast gaining attention as a key regulator of serum triglyceride concentrations. An ApoA5 mouse knock-out model produced an approximately four fold increase in serum triglycerides, whereas a knock-in model with human ApoA5 produced 50-70% lower concentrations of mouse serum triglycerides. In addition, peroxisome proliferator-activated receptor-_ agonists, which are used clinically to lower serum triglyceride concentrations, cause increased ApoA5 mRNA expression. Recently, it was demonstrated that ApoA5 is present in human serum detected by polyclonal antibodies against both the NH2 and COOH termini, although at much lower concentration than other apolipoproteins.

Specificity: Ni-NTA purified truncated recombinant human APOA5 expressed in E. Coli strain BL21 (DE3)

Application Details

Application Notes: Dilution: Western Blot: 1:500-1,000
ELISA: Determining optimal working dilutions by titration test.

Purification: Crude ascites.

Storage: Store at -20° C.

Restrictions: For Research Use only

Publications

Product: Pennacchio, Olivier, Hubacek et al.: "An apolipoprotein influencing triglycerides in humans and mice revealed by comparative sequencing." in: Science (New York, N.Y.), Vol. 294, Issue 5540, pp. 169-73, 2001 (PubMed).

Prieur, Coste, Rodriguez: "The human apolipoprotein AV gene is regulated by peroxisome proliferator-activated receptor-alpha and contains a novel farnesoid X-activated receptor response element." in: The Journal of biological chemistry, Vol. 278, Issue 28, pp. 25468-80, 2003 (PubMed).