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Activating Transcription Factor 2 (ATF2) antibody

Antigen

Activating Transcription Factor 2 (ATF2)
Synonyms HB16, CREB2, TREB7, CRE-BP1, MGC111558, ATF2, MGC142504, CHN1, CG12850, CG3749, dATF-2, DmelCG30420, CG30420
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN965613
Quantity 100ug  (100ug/100ul)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Alternative name ATF2
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human ATF-2 around the phosphorylation site of threonine 71 or 53 (T-P-TP-P-T).
Specificity ATF-2(Ab-71or 53) antibody detects endogenous levels of total ATF-2 protein.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Abdel-Hafiz, Heasley, Kyriakis et al.: "Activating transcription factor-2 DNA-binding activity is stimulated by phosphorylation catalyzed by p42 and p54 microtubule-associated protein kinases." in: Molecular endocrinology (Baltimore, Md.), Vol. 6, Issue 12, pp. 2079-89, 1993 (PubMed).

van Dam, Wilhelm, Herr et al.: "ATF-2 is preferentially activated by stress-activated protein kinases to mediate c-jun induction in response to genotoxic agents." in: The EMBO journal, Vol. 14, Issue 8, pp. 1798-811, 1995 (PubMed).

Gupta, Campbell, Dérijard et al.: "Transcription factor ATF2 regulation by the JNK signal transduction pathway." in: Science (New York, N.Y.), Vol. 267, Issue 5196, pp. 389-93, 1995 (PubMed).

Waetzig, Seegert, Rosenstiel et al.: "p38 mitogen-activated protein kinase is activated and linked to TNF-alpha signaling in inflammatory bowel disease." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 10, pp. 5342-51, 2002 (PubMed).

Sevilla, Santos, Vega et al.: "Human vaccinia-related kinase 1 (VRK1) activates the ATF2 transcriptional activity by novel phosphorylation on Thr-73 and Ser-62 and cooperates with JNK." in: The Journal of biological chemistry, Vol. 279, Issue 26, pp. 27458-65, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Activating Transcription Factor 2 (ATF2)", type "Antibodies"
Hosts Rabbit (260), Mouse (12), Goat (7), Sheep (7)
Reactivities Human (276), Rat (Rattus) (157), Mouse (Murine) (146), Cow (Bovine) (50), Horse (Equine) (43), Dog (Canine) (29), Chicken (22), Pig (Porcine) (20), Rabbit (18), Guinea Pig (5), Bird (Avian) (2), Cat (Feline) (1), Xenopus laevis (1)
Applications Western Blotting (WB) (158), Immunohistochemistry (IHC) (111), Immunofluorescence (IF) (94), ELISA (85), Immunoprecipitation (IP) (57), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (52), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunocytochemistry (ICC) (4), Electrophoretic Mobility-Shift Assay (EMSA) (3), Immunoelectron Microscopy (IEM) (2), Flow Cytometry (FACS) (1), Functional Studies (Func) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (9), Alexa Fluor 488 (9), Alexa Fluor 555 (9), Alexa Fluor 647 (9), PE,Cy5.5 (8), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1), Un-conjugated (1)
Epitopes Thr53 (12), pSer472 (9), pSer322 (8), pSer44 (8), pSer94 (8), C-Term (7), N-Term (7), pThr51 (7), pThr53 (6), pThr69, pThr71 (5), pThr71 (5), pSer498,pSer490 (4), pThr69, pThr51 (4), pSer112, pSer94 (3), pSer44, pSer62 (3), pSer490, pSer498 (3), pSer62, pSer44 (3), pSer62,pSer44 (3), pThr51, pThr69 (3), pThr53, pThr71 (3), pThr55, pThr73 (3), pThr69,pThr51 (3), pThr71, pThr53 (3), pThr71,pThr53 (3), pThr73, pThr55 (3), Ab-112 or 94 (2), Ab-62 or 44 (2), Ab-69 or 51 (2), Ab-71 or 53 (2), Ab-73 or 55 (2), Internal Region (2), Ser44 (2), Ser94 (2), pSer112 or 94 (2), pSer112,pSer94 (2), pSer62 or 44 (2), pSer94, pSer112 (2), pThr69 or 51 (2), pThr71 or 53 (2), pThr73 or 55 (2), pThr73,pThr55 (2), AA 13-27 (1), AA 95-211 (1), Phosphospecific (1), Ser112, Ser94 (1), Ser62 (1), Ser62, Ser44 (1), Ser69, Ser71 (1), Ser94, Ser112 (1), Thr51 (1), Thr71, Thr51 (1), Thr73, Thr55 (1), Thr99, Thr51 (1), pSer112 (1), pSer44,pSer62 (1), pSer480 (1), pSer490,pSer498 (1), pSer62 (1), pSer730 (1), pSer90 (1), pSer94, pSer119 (1), pSer94,pSer112 (1), pThr286 (1), pThr34 (1), pThr51,pThr69 (1), pThr53,pThr71 (1), pThr55,pThr73 (1), pThr69 (1), pThr69,pThr51,pThr71,pThr53 (1), pThr73 (1), pTyr412 (1)