BCL antibody

Catalog No. ABIN965649

Product Details

Target: BCL

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunohistochemistry (IHC)

Specificity: BCL-2(Ab-70) antibody detects endogenous levels of total BCL-2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: This antiserum was produced against synthesized non-phosphopeptide derived from human BCL-2 around the phosphorylation site of serine 70 (R-T-SP-P-L).

Application Details

Application Notes: IHC: 1:50-1:100.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Jin, Gao, Flagg, Deng: "Tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone promotes functional cooperation of Bcl2 and c-Myc through phosphorylation in regulating cell survival and proliferation." in: The Journal of biological chemistry, Vol. 279, Issue 38, pp. 40209-19, (2004) (PubMed).

Huang, Cidlowski: "Phosphorylation status modulates Bcl-2 function during glucocorticoid-induced apoptosis in T lymphocytes." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 16, Issue 8, pp. 825-32, (2002) (PubMed).

Yamamoto, Ichijo, Korsmeyer: "BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M." in: Molecular and cellular biology, Vol. 19, Issue 12, pp. 8469-78, (2000) (PubMed).

Target Details

Target: BCL