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Jun Proto-Oncogene (JUN) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN965721
Quantity 100ug  (100ug/100ul)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name c-Jun
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of tyrosine 170 (P-V-YP-A-N).
Specificity C-Jun (Ab-170) antibody detects endogenous levels of c-Jun protein around tyrosine 170.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Kyriakis, Banerjee, Nikolakaki et al.: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

Barilá, Mangano, Gonfloni et al.: "A nuclear tyrosine phosphorylation circuit: c-Jun as an activator and substrate of c-Abl and JNK." in: The EMBO journal, Vol. 19, Issue 2, pp. 273-81, 2000 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (451), Mouse (40), Chicken (1), Sheep (1)
Reactivities Human (487), Mouse (Murine) (327), Rat (Rattus) (317), Cow (Bovine) (190), Dog (Canine) (190), Pig (Porcine) (186), Chicken (168), Sheep (Ovine) (127), Horse (Equine) (90), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (303), Immunofluorescence (IF) (182), ELISA (137), Immunohistochemistry (IHC) (129), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (97), Immunoprecipitation (IP) (44), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (29), Immunocytochemistry (ICC) (15), Immunoelectron Microscopy (IEM) (10), Flow Cytometry (FACS) (8), Dot Blot (DB) (7), Dot Blot (Dot) (6), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Biotin (10), Cy3 (10), Cy5 (10), Cy5.5 (10), Cy7 (10), FITC (10), Gold (10), HRP (10), PE (10), PE,Cy3 (10), PE,Cy5 (10), PE,Cy7 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), PE-Cy5.5 (1)
Epitopes pSer73 (37), pSer63 (32), pThr239 (31), pThr91 (31), pTyr170 (31), pSer243 (30), C-Term (25), pThr249 (20), pThr91,pThr93 (17), pThr93,pThr91 (17), pThr93 (13), Internal Region (12), N-Term (7), Ser73 (5), Thr239 (4), Tyr170 (3), AA 266-285 (2), N-Term,AA 1-30 (2), Ser243 (2), Ser63 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr243 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,AA 229-259 (1), C-Term,AA 240-269 (1), C-Term,AA 264-293 (1), C-Term,AA 266-296 (1), C-Term,Glu248 (1), Internal Region,AA 208-238 (1), Internal Region,AA 75-105 (1), Internal Region,AA 79-108 (1), Internal Region,AA 79-109 (1), Middle Region (1), N-Term,AA 35-65 (1), Ser73,Phosphospecific (1), pSer58 (1), pThr231 (1), pThr289 (1), pThr8 (1), pThr89 (1), pThr95 (1)