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Jun Proto-Oncogene (JUN) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN965723
Quantity 100ug  (100ug/100ul)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name c-Jun
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of serine 243 (P-L-SP-PI).
Specificity C-Jun (Ab-243) antibody detects endogenous levels of total c-Jun protein.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100
Concentration 100ug/100ul
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
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Product Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).

Boyle, Smeal, Defize et al.: "Activation of protein kinase C decreases phosphorylation of c-Jun at sites that negatively regulate its DNA-binding activity." in: Cell, Vol. 64, Issue 3, pp. 573-84, 1991 (PubMed).

Binétruy, Smeal, Karin: "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain." in: Nature, Vol. 351, Issue 6322, pp. 122-7, 1991 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Kyriakis, Banerjee, Nikolakaki et al.: "The stress-activated protein kinase subfamily of c-Jun kinases." in: Nature, Vol. 369, Issue 6476, pp. 156-60, 1994 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (351), Mouse (9), Chicken (1), Sheep (1)
Reactivities Human (355), Mouse (Murine) (237), Rat (Rattus) (224), Cow (Bovine) (86), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (258), Immunohistochemistry (IHC) (124), ELISA (115), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (41), Immunocytochemistry (ICC) (13), Dot Blot (Dot) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Dot Blot (DB) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1), Immunohistochemistry (Frozen Sections) (IHC (fro)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (20), pThr93,pThr91 (17), pSer63 (16), pThr239 (16), pThr91 (15), pTyr170 (15), pSer243 (14), Internal Region (12), pThr93 (10), C-Term (7), N-Term (7), pThr249 (7), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr243 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)