Add to Basket Bulk discount
Order hotline:
+1 404 474 4654
+1 888 205 9894 (TF)

Jun Proto-Oncogene (JUN) antibody

Antigen

Jun Proto-Oncogene (JUN)
Synonyms AP1, AP-1, c-Jun, fj36h07, zgc:65863, wu:fj36h07, jun
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
6 references available
Catalog no. ABIN965725
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
Bulk discount
Shipping to
Availability Will be delivered in 5 to 7 Business Days

Additional Information
Application Details Publications Alternatives Variants back to top

Alternative name c-Jun
Immunogen This antiserum was produced against synthesized non-phosphopeptide derived from human c-Jun around the phosphorylation site of serine 73 (L-A-SP-P-E).
Specificity C-Jun (Ab-73) antibody detects endogenous levels of total c-Jun protein.

Application Details
Publications Alternatives Variants back to top

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Smeal, Binetruy, Mercola et al.: "Oncogenic and transcriptional cooperation with Ha-Ras requires phosphorylation of c-Jun on serines 63 and 73." in: Nature, Vol. 354, Issue 6353, pp. 494-6, 1992 (PubMed).

Binétruy, Smeal, Karin: "Ha-Ras augments c-Jun activity and stimulates phosphorylation of its activation domain." in: Nature, Vol. 351, Issue 6322, pp. 122-7, 1991 (PubMed).

Dérijard, Hibi, Wu et al.: "JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain." in: Cell, Vol. 76, Issue 6, pp. 1025-37, 1994 (PubMed).

Hook, Peeling: "Syphilis control--a continuing challenge." in: The New England journal of medicine, Vol. 351, Issue 2, pp. 122-4, 2004 (PubMed).

Beausoleil, Jedrychowski, Schwartz et al.: "Large-scale characterization of HeLa cell nuclear phosphoproteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, Issue 33, pp. 12130-5, 2004 (PubMed).

Sevilla, Santos, Barcia et al.: "c-Jun phosphorylation by the human vaccinia-related kinase 1 (VRK1) and its cooperation with the N-terminal kinase of c-Jun (JNK)." in: Oncogene, Vol. 23, Issue 55, pp. 8950-8, 2004 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "Jun Proto-Oncogene (JUN)", type "Antibodies"
Hosts Rabbit (348), Mouse (9), Chicken (1), Goat (1), Sheep (1)
Reactivities Human (350), Rat (Rattus) (197), Mouse (Murine) (189), Cow (Bovine) (87), Dog (Canine) (86), Pig (Porcine) (82), Chicken (77), Sheep (Ovine) (62), Horse (Equine) (25), Xenopus laevis (8), Rabbit (7), Monkey (5), Zebrafish (5), Fruit Fly (Drosophila melanogaster) (3), Guinea Pig (3), Hamster (3), Cat (Feline) (1)
Applications Western Blotting (WB) (254), Immunohistochemistry (IHC) (124), ELISA (118), Immunofluorescence (IF) (101), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (72), Immunoprecipitation (IP) (42), Dot Blot (Dot) (6), Immunocytochemistry (ICC) (6), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (3), Immunoelectron Microscopy (IEM) (2), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (10), Alexa Fluor 555 (10), Alexa Fluor 647 (10), Alexa Fluor 488 (9), PE,Cy5.5 (9), Biotin (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), HRP (2), PE (2), PE,Cy3 (2), PE,Cy5 (2), PE,Cy7 (2), PE-Cy5.5 (1)
Epitopes pSer73 (26), pSer63 (20), pThr239 (19), pTyr170 (18), pThr91 (17), pThr93,pThr91 (17), pSer243 (16), Internal Region (12), pThr93 (12), C-Term (8), pThr249 (8), N-Term (7), Ser73 (5), Thr239 (4), pThr91,pThr93 (4), Ser243 (3), Ser63 (3), Tyr170 (3), pThr243 (3), AA 266-285 (2), Thr243 (2), Thr249 (2), Thr91 (2), Thr93 (2), pThr91, pThr93 (2), AA 1-240 (1), AA 59-67 (1), C-Term,Glu248 (1), Middle Region (1), Ser73,Phosphospecific (1), Sumoylation Site (1), non-pSer73 (1), pSer213 (1), pSer539,pSer535 (1), pThr18 (1), pThr231 (1), pTyr981,pTyr980 (1)