CHD3 antibody

Catalog No. ABIN965879

The Mouse Monoclonal anti-CHD3 antibody has been validated for WB, IHC, ELISA, IF and FACS. It is suitable to detect CHD3 in samples from Human and Mouse. There are 3+ publications available.

Quick Overview for CHD3 antibody (ABIN965879)

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This CHD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 2G4

Product Details anti-CHD3 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CHD3 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CHD3 antibody (ABIN965879)

Burd, Kinyamu, Miller, Archer: "UV radiation regulates Mi-2 through protein translation and stability." in: The Journal of biological chemistry, Vol. 283, Issue 50, pp. 34976-82, (2008) (PubMed).

Goehler, Lalowski, Stelzl, Waelter, Stroedicke, Worm, Droege, Lindenberg, Knoblich, Haenig, Herbst, Suopanki, Scherzinger, Abraham, Bauer, Hasenbank, Fritzsche, Ludewig, Büssow, Buessow, Coleman et al.: "A protein interaction network links GIT1, an enhancer of huntingtin aggregation, to Huntington's disease. ..." in: Molecular cell, Vol. 15, Issue 6, pp. 853-65, (2004) (PubMed).

Lepesheva, Virus, Waterman: "Conservation in the CYP51 family. Role of the B' helix/BC loop and helices F and G in enzymatic function." in: Biochemistry, Vol. 42, Issue 30, pp. 9091-101, (2003) (PubMed).

Target Details for CHD3

Target: CHD3 (Chromodomain Helicase DNA Binding Protein 3 (CHD3))

Alternative Name: CHD3

Background: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described.
Synonyms: ZFH, Mi-2a, Mi2ALPHA, CHD3

Molecular Weight: 226kDa

Gene ID: 1107