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CAMP Responsive Element Binding Protein 1 (CREB1) antibody

Antigen

CAMP Responsive Element Binding Protein 1 (CREB1)

Synonyms CREB, MGC9284, Creb, Creb-1, AV083133, 2310001E10Rik, 3526402H21Rik, JAL, hJAL, ATCE1, CREB3, CREB4, AIBZIP, CREB1, creb1, MGC52669, creb, MGC69285, MGC55598, zgc:55598, Creb1, DKFZp468J2311
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
5 references available
Catalog no. ABIN965927
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name CREB
Immunogen This antiserum was produced against synthesized non-phosphopeptide derived from human CREB around the phosphorylation site of serine 133 (R-P-SP-Y-R).
Specificity CREB (Ab-133) antibody detects endogenous levels of total CREB protein.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Tan, Rouse, Zhang et al.: "FGF and stress regulate CREB and ATF-1 via a pathway involving p38 MAP kinase and MAPKAP kinase-2." in: The EMBO journal, Vol. 15, Issue 17, pp. 4629-42, 1997 (PubMed).

Hao, Lowy, Kapoor et al.: "Mutation of phosphoserine 389 affects p53 function in vivo." in: The Journal of biological chemistry, Vol. 271, Issue 46, pp. 29380-5, 1997 (PubMed).

Rosenfeld, Correia, Xing et al.: "Structural studies of kinesin-nucleotide intermediates." in: The Journal of biological chemistry, Vol. 271, Issue 47, pp. 30212-21, 1997 (PubMed).

Xing, Kornhauser, Xia et al.: "Nerve growth factor activates extracellular signal-regulated kinase and p38 mitogen-activated protein kinase pathways to stimulate CREB serine 133 phosphorylation." in: Molecular and cellular biology, Vol. 18, Issue 4, pp. 1946-55, 1998 (PubMed).

Westerheide, Mayo, Anest et al.: "The putative oncoprotein Bcl-3 induces cyclin D1 to stimulate G(1) transition." in: Molecular and cellular biology, Vol. 21, Issue 24, pp. 8428-36, 2001 (PubMed).

Alternatives

Alternatives for antigen "CAMP Responsive Element Binding Protein 1 (CREB1)", type "Antibodies"
Hosts Rabbit (230), Mouse (50)
Reactivities Human (265), Rat (Rattus) (199), Mouse (Murine) (188), Pig (Porcine) (111), Sheep (Ovine) (110), Cow (Bovine) (97), Chicken (96), Dog (Canine) (61), Horse (Equine) (54), Monkey (5), Xenopus laevis (4), Zebrafish (2), Zebrafish (Danio rerio) (2), Cat (Feline) (1)
Applications Western Blotting (WB) (160), Immunofluorescence (IF) (111), ELISA (83), Immunohistochemistry (IHC) (69), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (57), Immunoprecipitation (IP) (23), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (18), Immunocytochemistry (ICC) (12), Flow Cytometry (FACS) (6), Immunoelectron Microscopy (IEM) (6), Immunohistochemistry (Fixed) (IHC (fx)) (2), Dot Blot (Dot) (1), Electrophoretic Mobility-Shift Assay (EMSA) (1), Gel Shift (GS) (1)
Conjugates Biotin (9), Alexa Fluor 488 (7), Alexa Fluor 350 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), Cy3 (6), Cy5 (6), Cy5.5 (6), Cy7 (6), FITC (6), Gold (6), HRP (6), PE (6), PE,Cy3 (6), PE,Cy5 (6), PE,Cy5.5 (6), PE,Cy7 (6)
Epitopes pSer133 (59), pSer129 (33), pSer142 (22), pSer121 (19), N-Term (8), Internal Region (4), Ser129 (4), Ser133 (4), Center (2), pS133 (2), phosphospecific (2), AA 1-96 (1), AA 100-150 (1), AA 112-140 (1), AA 122-147,pSer133 (1), AA 136-341 (1), AA 152-179 (1), AA 5-24 (1), AA 5-45 (1), Ser142 (1), pSer129, pSer133 (1), pThr100 (1)