Cytokeratin18 (45kDa) antibody

Antigen: Cytokeratin18 (45kDa)

Clonality: Monoclonal (B23-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Catalog no.: ABIN965968

Additional Information

Gene ID: 3875

Immunogen: PMC-42 Human Breast Carcinoma Cells

Isotype: IgG1

Clone: B23-1

Description: This antibody reacts with Cytokeratin 18 (45 kD) polypeptide and with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid.

Comments:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Colon Carcinoma

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen).
Staining Procedure: When using paraffin-embedded and formalin-fixed tissues, predigestion with AutoZyme or high temperature antigenic unmasking technique is strongly recommended for consistent and reproducible results. The optimal conditions should be determined by the individual laboratory. The antibody may be used at a dilution of 1:50 to 1:100 with AutoProbe III.

Concentration: 0.3 mg / ml.

Purification: Concentrated.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: 0.05% Sodium Azide

Storage: Store at 2-8° C for short term, freeze under -20°C for long term storage.

Restrictions: For Research Use only

Publications

Product: Moll, Franke, Schiller et al.: "The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells." in: Cell, Vol. 31, Issue 1, pp. 11-24, 1983 (PubMed).