ERCC2 antibody (C-Term)

Catalog No. ABIN966088

This Rabbit Polyclonal antibody specifically detects ERCC2 in IHC. It exhibits reactivity toward Human and Mouseand has been mentioned in 1 publication.

Quick Overview for ERCC2 antibody (C-Term) (ABIN966088)

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERCC2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-ERCC2 Antibody

Binding Specificity: C-Term

Purification: Purified by antigen-specific affinity chromatography.

Immunogen: Polyclonal antibody produced in rabbits immunizing with a synthetic peptide corresponding to C-terminal residues of human ERCC2(TFIIH basal transcription factor complex helicase subunit)

Application Details

Application Notes: ELISA, Western blotting: 1µg/ml for 2hrs.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: This antibody is stored in PBS, 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-ERCC2 antibody (ABIN966088)

Coin, Marinoni, Rodolfo, Fribourg, Pedrini, Egly: "Mutations in the XPD helicase gene result in XP and TTD phenotypes, preventing interaction between XPD and the p44 subunit of TFIIH." in: Nature genetics, Vol. 20, Issue 2, pp. 184-8, (1998) (PubMed).

Target Details for ERCC2

Target: ERCC2 (Excision Repair Cross-Complementing Rodent Repair Deficiency, Complementation Group 2 (ERCC2))

Alternative Name: ERCC2

Background: ERCC2(TFIIH basal transcription factor complex helicase subunit) is an ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. ERCC2 is involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. ERCC2 might also have a role in aging process and could play a causative role in the generation of skin cancers. One of the six subunits forming the core-TFIIH basal transcription factor. The interaction with p44 results in the stimulation of the 5'-->3' helicase activity. Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D), xeroderma pigmentosum group D combined with Cockayne syndrome (XP-D/CS). Defects in ERCC2 are a cause of trichothiodystrophy (TTD) and COFS syndrome. ERCC2 belongs to the helicase family and RAD3/XPD subfamily.

Pathways: DNA Damage Repair