Glucokinase (Hexokinase 4) (GCK) antibody

Antigen: Glucokinase (Hexokinase 4) (GCK)

Synonyms: GK, GLK, HK4, HHF3, HKIV, HXKP, MODY2, Gk, GCK, MGC166120, glk, hk4, hhf3, hkiv, hxkp, gck-A, mody2, glucokinase

Clonality: Monoclonal (4G6)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Catalog no.: ABIN966192

Additional Information

Alternative name: GCK

Gene ID: 2645

Immunogen: Purified recombinant fragment of human GCK expressed in E. Coli.

Isotype: IgG1

Clone: 4G6

Description: Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. Alternative splicing of this gene results in three tissue-specific forms of glucokinase, one found in pancreatic islet beta cells and two found in liver. The protein localizes to the outer membrane of mitochondria. In contrast to other forms of hexokinase, this enzyme is not inhibited by its product glucose-6-phosphate but remains active while glucose is abundant. Mutations in this gene have been associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young, type 2 (MODY2) and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
Synonyms: GK, GLK, HK4, HHF3, HKIV, HXKP, LGLK, MODY2, FGQTL3

Molecular Weight: 52kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Restrictions: For Research Use only

Publications

Product: Yoshida, Kato, Yokoi et al.: "Association of genetic variants with chronic kidney disease in individuals with different lipid profiles." in: International journal of molecular medicine, Vol. 24, Issue 2, pp. 233-46, 2009 (PubMed).

Barbetti, Cobo-Vuilleumier, Dionisi-Vici et al.: "Opposite clinical phenotypes of glucokinase disease: Description of a novel activating mutation and contiguous inactivating mutations in human glucokinase (GCK) gene." in: Molecular endocrinology (Baltimore, Md.), Vol. 23, Issue 12, pp. 1983-9, 2009 (PubMed).