Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN966238

GST-Tag antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Host
Clonality Monoclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Western Blotting (WB), ELISA
Pubmed 1 reference available
Catalog no. ABIN966238
Quantity 0.1 mg
Price
177.38 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 5 to 7 Business Days
Isotype IgG1
Specificity Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Purification Antibodies are purified by protein A affinity chromatography
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Research Area Bacteria, Tags/Labels
Application Notes Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Product cited in: Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, 1988 (PubMed).

Alternatives for antigen "GST-Tag", type "Antibodies"
Hosts (52), (51), (35), (4), (3)
Reactivities (91), (37), (10), (7), (6), (6), (4), (1), (1), (1), (1)
Applications (119), (83), (47), (24), (19), (16), (10), (9), (7), (6), (6), (2), (1), (1), (1), (1)
Conjugates (14), (7), (6), (4), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (2)
Beacon