GST-Tag antibody

Details for Product No. ABIN966238
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Reactivity
Tag
(100), (24), (8), (6), (6), (5), (5), (4), (4), (2), (1), (1)
Host
Please inquire
(65), (49), (27), (3), (2), (2)
Clonality
Monoclonal
Conjugate
Un-conjugated
(12), (7), (5), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), ELISA
(126), (68), (44), (19), (15), (14), (11), (10), (7), (6), (5), (4), (1), (1), (1)
Pubmed 1 reference available
Quantity 0.1 mg
Options
Shipping to United States (Change)
Availability Will be delivered in 5 to 7 Business Days
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Catalog No. ABIN966238
177.38 $
Plus shipping costs $45.00

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Isotype IgG1
Specificity Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
Purification Antibodies are purified by protein A affinity chromatography
Target Type Tag
Background Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
Application Notes Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.
Restrictions For Research Use only
Storage -20 °C
Product cited in: Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, 1988 (PubMed).

Validation Images
Did you look for something else?
back to top