GST-Tag antibody
| Antigen |
|
| Clonality |
Monoclonal |
|
Host
|
|
|
Reactivity
|
|
|
Conjugate
|
|
|
Application
|
Western Blotting (WB) (69), ELISA (43), Immunoprecipitation (IP) (22), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (6), Flow Cytometry (FACS) (4), Immunofluorescence (IF) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (DB) (2), Dot Blot (Dot) (2), Immunostaining (ISt) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
|
| Catalog no. |
ABIN966238 |
| Quantity |
0.1mg |
| Price |
177.38 $ Plus shipping costs $45.00
|
|
Bulk discount
|
| Shipping to |
|
| Availability |
Will be delivered in 5 to 7 Business Days |
|
Alternative name
|
GST Tag
|
|
Isotype
|
IgG1
|
|
Description
|
Plasmid vectors for the expression of coding regions of eukaryotic genes in E. coliare in common usage, such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. For instance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. coli.
|
|
Specificity
|
Ni-NTA purified recombinant GST expressed in E. Coli strain M15.
|
|
Application Notes
|
Western Blot: Dilution 1: 2000- 5,000
ELISA: Proposed dilution 1: 10,000.
Determining optimal working dilutions by titration test.
|
|
Purification
|
Antibodies are purified by protein A affinity chromatography
|
|
Storage
|
Store at -20° C.
|
|
Research Area
|
Bacteria, Tags/Labels
|
|
Restrictions
|
For Research Use only
|
|
Product
|
Smith, Johnson: "Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase." in: Gene, Vol. 67, Issue 1, pp. 31-40, 1988 (PubMed).
|
Alternatives for antigen "GST-Tag", type "Antibodies"
|
Hosts
|
Goat (28), Mouse (27), Rabbit (17), Rat (3), Chicken (2)
|
|
Reactivities
|
Schistosoma japonicum (35), Human (7), All Species (6), Mouse (Murine) (5), Rat (Rattus) (5), Tag (5), Bacteria (1), Escherichia coli (E. coli) (1), Mammalian (1), Rabbit (1)
|
|
Applications
|
Western Blotting (WB) (69), ELISA (43), Immunoprecipitation (IP) (22), Immunohistochemistry (IHC) (11), Immunocytochemistry (ICC) (6), Flow Cytometry (FACS) (4), Immunofluorescence (IF) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (4), Dot Blot (DB) (2), Dot Blot (Dot) (2), Immunostaining (ISt) (2), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (1)
|
|
Conjugates
|
HRP (6), Biotin (3), FITC (3), Agarose Beads (2), Alkaline Phosphatase (AP) (1), TRITC (1), Texas Red (TR) (1)
|
Alternatives Un-conjugated 
2013 BioMed Central Research Awards
