Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase 4 (MAP2K4)

Synonyms: JNKK, MEK4, MKK4, SEK1, JNKK1, SERK1, MAPKK4, PRKMK4, Sek1, Serk1, map2k4a, MGC136500, zgc:136500, jnkk, mek4, mkk4, sek1, jnkk1, serk1, xmek2, mapkk4, prkmk4, 142758_at, CT27508, D-MKK4, DMKK4, JNKK2, Mpk4, SEK1/MKK4, DmelCG9738, CG9738, CT38386, DmelCG12798, CG12798, DmelCR12798

Clonality: Monoclonal

Host: Please enquire

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Catalog no.: ABIN966514

Additional Information

Alternative name: MAP2K4

Gene ID: 6416

Isotype: IgM

Description: MAP2K4(mitogen-activated protein kinase kinase 4), which is located on chromosome 17p11.2, with 399-amino acid protein (about 45 kDa), belongs to the family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. MEK-4 (also called MEK4/MKK4) activates both p38 and JNK MAP kinases. MKK4 is a central mediator in the stress activated protein kinase signaling pathway that responds to a number of cellular and environmental stressors. By phosphorylating MAP kinases such as JNK,MKK4 can ultimately transmit stress signals to nuclear transcription factors that mediate various processes including proliferation, apoptosis, and differentiation. Its distinct biological functions have been identified for MKK4 including a role in development, hepatogenesis, and metastasis suppression.

Specificity: Ni-NTA purified truncated recombinant MAP2K4 expressed in E. Coli strain BL21 (DE3)

Synonyms: JNKK, MEK4, MKK4, SEK1, JNKK1, SERK1, MAPKK4, PRKMK4, Sek1, Serk1, map2k4a, MGC136500, zgc:136500, jnkk, mek4, mkk4, sek1, jnkk1, serk1, xmek2, mapkk4, prkmk4, 142758_at, CT27508, D-MKK4, DMKK4, JNKK2, Mpk4, SEK1/MKK4, DmelCG9738, CG9738, CT38386, DmelCG12798, CG12798, DmelCR12798

Application Details

Application Notes: Western Blot: 1: 500- 1: 1,000
IHC(P): 1: 500- 1: 1,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Purification: Antibodies are purified by protein A affinity chromatography

Storage: Store at -20° C.

Restrictions: For Research Use only

Publications

Product: Crews, Alessandrini, Erikson: "The primary structure of MEK, a protein kinase that phosphorylates the ERK gene product." in: Science (New York, N.Y.), Vol. 258, Issue 5081, pp. 478-80, 1992 (PubMed).

Cuenda: "Mitogen-activated protein kinase kinase 4 (MKK4)." in: The international journal of biochemistry & cell biology, Vol. 32, Issue 6, pp. 581-7, 2000 (PubMed).

Kim, Vander Griend, Yang et al.: "Mitogen-activated protein kinase kinase 4 metastasis suppressor gene expression is inversely related to histological pattern in advancing human prostatic cancers." in: Cancer research, Vol. 61, Issue 7, pp. 2833-7, 2001 (PubMed).