Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) antibody

Antigen: Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5)

Synonyms: ASK1, MEKK5, MAPKKK5, MAP3K5, Ask1, mitogen-activated protein kinase kinase kinase 5, MUD21.11, MUD21_11

Clonality: Monoclonal (2E4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), ELISA

Catalog no.: ABIN966515

Additional Information

Alternative name: MAP3K5

Gene ID: 4217

Immunogen: Purified recombinant fragment of human MAP3K5 expressed in E. Coli.

Isotype: IgG1

Clone: 2E4

Description: Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun Nterminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Synonyms: ASK1, MEKK5, MAPKKK5

Molecular Weight: 55kDa

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Purification: Ascitic fluid

Preservative: 0.03% sodium azide.

Storage: Store at 4°C, for long term storage, store at -20°C.

Restrictions: For Research Use only

Publications

Product: Woolcott, SenGupta, Hanna et al.: "Estrogen and progesterone receptor levels in nonneoplastic breast epithelium of breast cancer cases versus benign breast biopsy controls." in: BMC cancer, Vol. 8, pp. 130, 2008 (PubMed).

Widenmaier, Ao, Kim et al.: "Suppression of p38 MAPK and JNK via Akt-mediated inhibition of apoptosis signal-regulating kinase 1 constitutes a core component of the beta-cell pro-survival effects of glucose-dependent insulinotropic polypeptide." in: The Journal of biological chemistry, Vol. 284, Issue 44, pp. 30372-82, 2009 (PubMed).

Pressinotti, Klocker, Schäfer et al.: "Differential expression of apoptotic genes PDIA3 and MAP3K5 distinguishes between low- and high-risk prostate cancer." in: Molecular cancer, Vol. 8, pp. 130, 2010 (PubMed).