MTDH antibody

Catalog No. ABIN966573

Cited in 4+ publications. The Mouse Monoclonal anti-MTDH antibody (Clone 2F11C3) (ABIN966573) specifically detects MTDH in WB, IHC, ELISA and FACS. The antibody is reactive with Human samples.

Quick Overview for MTDH antibody (ABIN966573)

Target: MTDH (Metadherin (MTDH))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MTDH antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Clone: 2F11C3

Product Details anti-MTDH Antibody

Purification: Antibodies are purified by protein G affinity chromatography.

Immunogen: Purified recombinant fragment of human Metadherin expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 50% glycerol

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MTDH antibody (ABIN966573)

Hu, Chong, Yang, Wei, Blanco, Li, Reiss, Au, Haffty, Kang: "MTDH activation by 8q22 genomic gain promotes chemoresistance and metastasis of poor-prognosis breast cancer." in: Cancer cell, Vol. 15, Issue 1, pp. 9-20, (2008) (PubMed).

Méndez, Audicana, Cancer, Isern, Llaneza, Moreno, Navarro, Tarancón, Valero, Ribas, Jofre, Lucena: "Assessment of drinking water quality using indicator bacteria and bacteriophages." in: Journal of water and health, Vol. 2, Issue 3, pp. 201-14, (2004) (PubMed).

Britt, Yang, Yang, Flanagan, Callanan, Lim, Lin, Hixson: "Identification of a novel protein, LYRIC, localized to tight junctions of polarized epithelial cells." in: Experimental cell research, Vol. 300, Issue 1, pp. 134-48, (2004) (PubMed).

Brown, Ruoslahti: "Metadherin, a cell surface protein in breast tumors that mediates lung metastasis." in: Cancer cell, Vol. 5, Issue 4, pp. 365-74, (2004) (PubMed).

Target Details for MTDH

Target: MTDH (Metadherin (MTDH))

Alternative Name: Metadherin

Background: Metadherin (Metastasis adhesion protein), also known as MTDH, LYsine-RIch CEACAM1 co-isolated (LYRIC), is a novel protein that localizes with the tight junction proteins ZO-1 and occludin in polarized epithelial cells. At the tight junction, it acts not as a structural component, but is rather recruited during the maturation of the tight junction complex. Metadherin is overexpressed in breast cancer tissue and breast tumor xenografts, while much lower levels are expressed in normal breast tissue. Metadherin binds to lung vasculature, one of the four common sites of breast cancer metastasis, through a C-terminal segment in the extracellular domain, blocking this lung-homing domain with antibodies or inhibiting metadherin with siRNA has been reported to inhibit breast cancer metastasis.
Synonyms: 3D3, AEG1, LYRIC, MTDH

Molecular Weight: 64kDa

Gene ID: 92140

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization