P21-Activated Kinases 1/2/3 (PAK1/2/3) (pThr423,pThr402,pThr421) antibody

Antigen: P21-Activated Kinases 1/2/3 (PAK1/2/3)

Synonyms: PAKalpha, MGC130000, MGC130001, Paka, PAK-1, AW045634, PAK1, MGC83445, MGC139676

Binding Site: pThr423,pThr402,pThr421

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Mouse (Murine), Rat (Rattus)

Application: Immunohistochemistry (IHC)

Catalog no.: ABIN966798

Additional Information

Alternative name: PAK1/PAK2/PAK3

Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human PAK1 around the phosphorylation site of threonine 423 (R-S-TP-M-V).

Specificity: PAK1/PAK2/PAK3 (Phospho-Thr423/Thr402/Thr421) antibody detects endogenous levels of PAK1/PAK2/PAK3 only when phosphorylated at threonine 423/402/421.

Application Details

Application Notes: IHC: 1:50-1:100.

Concentration: 100ug/100ul.

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol

Preservative: 0.02% Sodium Azide

Storage: Store at -20°C/1 year.

Restrictions: For Research Use only

Publications

Product: Lampugnani, Zanetti, Breviario et al.: "VE-cadherin regulates endothelial actin activating Rac and increasing membrane association of Tiam." in: Molecular biology of the cell, Vol. 13, Issue 4, pp. 1175-89, 2002 (PubMed).

Wang, Mazumdar, Vadlamudi et al.: "P21-activated kinase-1 phosphorylates and transactivates estrogen receptor-alpha and promotes hyperplasia in mammary epithelium." in: The EMBO journal, Vol. 21, Issue 20, pp. 5437-47, 2002 (PubMed).

Parsons, Monypenny, Ameer-Beg et al.: "Spatially distinct binding of Cdc42 to PAK1 and N-WASP in breast carcinoma cells." in: Molecular and cellular biology, Vol. 25, Issue 5, pp. 1680-95, 2005 (PubMed).

Jung, Traugh: "Regulation of the interaction of Pak2 with Cdc42 via autophosphorylation of serine 141." in: The Journal of biological chemistry, Vol. 280, Issue 48, pp. 40025-31, 2005 (PubMed).