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Paxillin (PXN) antibody

Antigen

Paxillin (PXN)

Synonyms FLJ16691, Pax, AW108311, AW123232, CG18061, CG18576, CT40481, CT42454, DPaxillin, DPxn, DPxn37, Dpax, PDLP, DmelCG31794, CG31794, LOC397826, wu:fw71f12, PXN, DKFZp469J2119, LOC100220858
Clonality Polyclonal
Host
Alternatives

Rabbit

Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)

Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
3 references available
Catalog no. ABIN966809
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information

Alternative name Paxillin
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Paxillin around the phosphorylation site of tyrosine 118 (H-V-YP-S-F).
Specificity Paxillin (Ab-118) antibody detects endogenous levels of total Paxillin protein.

Application Details

Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications

Product Rush, Moritz, Lee et al.: "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells." in: Nature biotechnology, Vol. 23, Issue 1, pp. 94-101, 2005 (PubMed).

Sanders, Basson: "p130cas but not paxillin is essential for Caco-2 intestinal epithelial cell spreading and migration on collagen IV." in: The Journal of biological chemistry, Vol. 280, Issue 25, pp. 23516-22, 2005 (PubMed).

Tao, Wollscheid, OBrien et al.: "Quantitative phosphoproteome analysis using a dendrimer conjugation chemistry and tandem mass spectrometry." in: Nature methods, Vol. 2, Issue 8, pp. 591-8, 2005 (PubMed).

Alternatives

Alternatives for antigen "Paxillin (PXN)", type "Antibodies"
Hosts Rabbit (145), Mouse (18), Sheep (1)
Reactivities Human (151), Mouse (Murine) (74), Rat (Rattus) (73), Dog (Canine) (24), Cow (Bovine) (23), Horse (Equine) (23), Chicken (12), Monkey (1), Xenopus laevis (1), Zebrafish (Danio rerio) (1)
Applications Western Blotting (WB) (105), Immunofluorescence (IF) (62), Immunohistochemistry (IHC) (34), ELISA (33), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (31), Immunocytochemistry (ICC) (15), Immunoprecipitation (IP) (10), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (5), Flow Cytometry (FACS) (2), Dot Blot (Dot) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Fixed) (IHC (fx)) (1)
Conjugates Alexa Fluor 350 (6), Alexa Fluor 488 (6), Alexa Fluor 555 (6), Alexa Fluor 647 (6), PE,Cy5.5 (6), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr118 (18), pTyr31 (16), pTyr88 (10), Tyr118 (5), pSer178 (5), pSer83 (5), C-Term (4), AA 356-558 (3), AA 59-274 (2), N-Term (2), Tyr31 (2), Tyr88 (2), pSer126 (2), AA 112-126 (1), AA 305-559 (1), Center (1), Internal Region (1), pSer17 (1), pSer23 (1), pThr383,pThr382,pSer380 (1), pTyr181 (1), pTyr753 (1)