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V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC) antibody

Antigen

V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC)
Synonyms ASV, SRC1, c-SRC, p60-Src, SDR, PP60C-SCR, SRC
Clonality Polyclonal
Host
Alternatives

Rabbit
Reactivity
Alternatives

Human, Mouse (Murine), Rat (Rattus)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Immunohistochemistry (IHC)
4 references available
Catalog no. ABIN967082
Quantity 100ug  (100ug/100ul.)  (Variants)
Price 385.00 $   Plus shipping costs $45.00
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Additional Information
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Alternative name Src
Immunogen The antiserum was produced against synthesized non-phosphopeptide derived from human Src around the phosphorylation site of tyrosine 529 (P-Q-YP-Q-P).
Specificity Src (Ab-529) antibody detects endogenous levels of total Src protein.

Application Details
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Application Notes WB: 1:500~1:1000
IHC: 1:50~1:100.
Concentration 100ug/100ul.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Buffer Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 50% glycerol
Preservative 0.02% Sodium Azide
Storage Store at -20°C/1 year.
Restrictions For Research Use only

Publications
Application Details Alternatives Variants back to top

Product Pyper, Bolen: "Identification of a novel neuronal C-SRC exon expressed in human brain." in: Molecular and cellular biology, Vol. 10, Issue 5, pp. 2035-40, 1990 (PubMed).

Parker, Mardon, Lebo et al.: "Isolation of duplicated human c-src genes located on chromosomes 1 and 20." in: Molecular and cellular biology, Vol. 5, Issue 4, pp. 831-8, 1985 (PubMed).

Xu, Harrison, Eck: "Three-dimensional structure of the tyrosine kinase c-Src." in: Nature, Vol. 385, Issue 6617, pp. 595-602, 1997 (PubMed).

Benes, Wu, Elia et al.: "The C2 domain of PKCdelta is a phosphotyrosine binding domain." in: Cell, Vol. 121, Issue 2, pp. 271-80, 2005 (PubMed).

Alternatives
Application Details Publications Variants back to top

Alternatives for antigen "V-Src Sarcoma (Schmidt-Ruppin A-2) Viral Oncogene Homolog (Avian) (SRC)", type "Antibodies"
Hosts Rabbit (110), Mouse (24)
Reactivities Human (129), Rat (Rattus) (79), Mouse (Murine) (67), Dog (Canine) (23), Cow (Bovine) (18), Chicken (13), Pig (Porcine) (10), Horse (Equine) (5), Sheep (Ovine) (5), Frog (1)
Applications Western Blotting (WB) (98), ELISA (46), Immunohistochemistry (IHC) (44), Immunofluorescence (IF) (34), Immunoprecipitation (IP) (11), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (9), Immunocytochemistry (ICC) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (Dot) (1), Flow Cytometry (FACS) (1), Immunoelectron Microscopy (IEM) (1)
Conjugates Alexa Fluor 350 (4), Alexa Fluor 488 (4), Alexa Fluor 555 (4), Alexa Fluor 647 (4), PE,Cy5.5 (4), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy7 (1)
Epitopes pTyr529 (16), pTyr418 (13), N-Term (10), pTyr215 (6), Tyr529 (3), pTyr216,Internal Region (3), pTyr416 (3), AA 10-193 (1), AA 519-536 (1), AA 6-19 (1), AA 92-109 (1), C-Term (1), Phosphospecific (1), Tyr215 (1), Tyr416 (1), pSer179 (1), pSer25 (1), pSer425,pSer423 (1), pSer75 (1), pTyr216 (1), pTyr419 (1), pTyr522 (1), pTyr527 (1), pY529 (1)