WIF1 antibody

Catalog No. ABIN967258

This Mouse Monoclonal antibody specifically detects WIF1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human and has been mentioned in 2+ publications.

Quick Overview for WIF1 antibody (ABIN967258)

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This WIF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 1G5

Product Details anti-WIF1 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human WIF1 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Immunofluorescence: 1/200 - 1/1000.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-WIF1 antibody (ABIN967258)

Veeck, Wild, Fuchs, Schüffler, Hartmann, Knüchel, Dahl: "Prognostic relevance of Wnt-inhibitory factor-1 (WIF1) and Dickkopf-3 (DKK3) promoter methylation in human breast cancer." in: BMC cancer, Vol. 9, pp. 217, (2009) (PubMed).

Kawakami, Hirata, Yamamura, Kikuno, Saini, Majid, Tanaka, Kawamoto, Enokida, Nakagawa, Dahiya: "Functional significance of Wnt inhibitory factor-1 gene in kidney cancer." in: Cancer research, Vol. 69, Issue 22, pp. 8603-10, (2009) (PubMed).

Target Details for WIF1

Target: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Alternative Name: WIF1

Background: The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. This protein contains a WNT inhibitory factor (WIF) domain and five epidermal growth factor (EGF)-like domains, and is thought to be involved in mesoderm segmentation. This gene functions as a tumor suppressor gene, and has been found to be epigenetically silenced in various cancers.
Synonyms: WIF-1, WIF

Molecular Weight: 42kDa

Gene ID: 11197

Pathways: WNT Signaling, Positive Regulation of fat Cell Differentiation