Spleen tyrosine Kinase (SYK) antibody

Antigen: Spleen tyrosine Kinase (SYK)

Synonyms: FLJ25043, FLJ37489, DKFZp313N1010, Syk, p72syk

Clonality: Monoclonal (4D10)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Catalog no.: ABIN967352

Additional Information

Alternative name: Syk

Components: 1) 51-16626N: EB1 Control Lysate.
Quantity: 50 µg (1 ea).
Clone: 4D10.
Immunogen: Human Syk peptide aa. 314-339.
Isotype: Mouse IgG2a, kappa.
Storage Buffer: SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

2) 51-8116GR: Purified Mouse Anti-Human Sy.
Quantity: 50 µg (3 ea).
Storage Buffer: Aqueous buffered solution containing BSA, glycerol, and Less or equal than 0.09% sodium azide).

Format: Liquid

Isotype: IgG2a

Clone: 4D10

Description: Syk is a member of the family of non-receptor type protein-tyrosine kinases and plays a crucial role in lymphocyte signaling and development. Syk is expressed in all haematopoietic cells and contributes to the signal transduction process by binding to a tyrosine-based activation motif (ITAM) of immune receptors, including Igalpha, TCRzeta, CD3epsilon, FcepsilonRIbeta, and FceRIgamma. Upon receptor activation, Syk binds to phosphorylated ITAMs via its two N-terminal SH2 domains thereby activating Syk and causing tyrosines in Syk to undergo auto-phosphorylation or phosphorylation. These phosphorylated sites can act as binding sites for other signaling molecules or help to regulate enzymatic activity. For example, in mast cells, Syk can activate downstream targets such as phospholipase Cgamma1 and VAV. Studies in Syk-/- mast cells identified defects in both the ERK-MAP and JNK-MAP kinase pathways. Syk migrates as 72 kDa in SDS/PAGE. (Predicted MW from SWISS-PROT is 72 kDa, Accession number P43405).

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Comments:

Related Products: ABIN967389

Application Details

Application Notes: Western Blot: EB1 control lysate [50 µg (1 µg/ml)] is provided as a positive control (store lysate at -20°C).
Additional application: The FITC-conjugated format of clone 4D10 is useful in immunofluorescent staining and flow cytometric analysis.

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Storage: Store undiluted at -20°C.

Research Area: Immunology, Innate Immunity, Tyrosine Kinases, Signaling

Restrictions: For Research Use only

Images

Western blot analysis of Syk. Lysate from EB1 cells was probed with anti-Syk (clone 4D10) at concentrations of 0.125 (lane 1), 0.063 (lane 2), and 0.031 µg/ml (lane 3). Syk is identified as a band of ~72 kDa.

Publications

Product: Costello, Turner, Walters et al.: "Critical role for the tyrosine kinase Syk in signalling through the high affinity IgE receptor of mast cells." in: Oncogene, Vol. 13, Issue 12, pp. 2595-605, 1997 (PubMed).

Chu, van Oers, Malissen et al.: "Pre-T cell receptor signals are responsible for the down-regulation of Syk protein tyrosine kinase expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 5, pp. 2610-20, 1999 (PubMed).

Turner, Schweighoffer, Colucci et al.: "Tyrosine kinase SYK: essential functions for immunoreceptor signalling." in: Immunology today, Vol. 21, Issue 3, pp. 148-54, 2000 (PubMed).

Latour, Veillette: "Proximal protein tyrosine kinases in immunoreceptor signaling." in: Current opinion in immunology, Vol. 13, Issue 3, pp. 299-306, 2001 (PubMed).