You are viewing an incomplete version of our website. Please click to reload the website as full version.

N-terminal Asparagine Amidase (NTAN1) antibody

Details for Product No. ABIN967387, Supplier: Login to see New
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Synonyms PNAA, PNAD
Mouse (Murine)
(26), (5)
(21), (5), (1)
Clonality (Clone)
Monoclonal ()
(4), (2), (2), (2), (2), (2)
Blocking Reagent (BR), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Zinc-fixed Sections) (IHC (zinc)), Immunoprecipitation (IP), Western Blotting (WB)
(20), (14), (4), (1), (1), (1)
Pubmed 9 references available
Supplier Login to see New
Catalog number from supplier Login to see New
Quantity 0.5 mg
Shipping to United States ( )
Brand BD Pharmingen™
Immunogen Collagenase-dispersed BALB/c lymph node stroma
Clone MECA-79
Isotype IgM, kappa
Specificity Carbohydrate Epitope
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name PNAd (NTAN1 Antibody Abstract)
Background The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin (eg, CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo, and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.
Synonyms: CD62L Ligand
Application Notes This antibody has been tested by immunohistochemical staining (IHC) of citrate-pretreated formalin-fixed paraffin-embedded sections (5 - 20 µg/ml) to assure specificity and reactivity. Other reported applications include IHC of acetone-fixed frozen sections, immunoprecipitation, western blot analysis, and in vitro and in vivo adhesion blocking.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Aqueous buffered solution containing ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store undiluted at 4°C.
Product cited in: Binns, Whyte, Licence et al.: "The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 4094-9, 1996 (PubMed).

Malý, Thall, Petryniak et al.: "The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis." in: Cell, Vol. 86, Issue 4, pp. 643-53, 1996 (PubMed).

Diacovo, Puri, Warnock et al.: "Platelet-mediated lymphocyte delivery to high endothelial venules." in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 252-5, 1996 (PubMed).

Michie, Streeter, Bolt et al.: "The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1688-98, 1994 (PubMed).

Faveeuw, Gagnerault, Lepault: "Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5969-78, 1994 (PubMed).

Puri, Finger, Gaudernack et al.: "Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules." in: The Journal of cell biology, Vol. 131, Issue 1, pp. 261-70, 1995 (PubMed).

Hemmerich, Butcher, Rosen: "Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody." in: The Journal of experimental medicine, Vol. 180, Issue 6, pp. 2219-26, 1994 (PubMed).

Streeter, Rouse, Butcher: "Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes." in: The Journal of cell biology, Vol. 107, Issue 5, pp. 1853-62, 1988 (PubMed).

Berg, Robinson, Warnock et al.: "The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 343-9, 1991 (PubMed).

Catalog No. ABIN967387
Contact our Customer Service for availability and price in your country.

Order hotline:

  • +1 877 302 8632
  • +1 888 205 9894 (TF)