N-terminal Asparagine Amidase (NTAN1) antibody

Details for Product No. ABIN967387
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

 
Antigen
Synonyms PNAA, PNAD
Reactivity
Mouse (Murine)
(6), (1)
Host
Rat
(4), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1)
Application
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (ffpe)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB), Blocking Antibody (Inhibition), Immunocytochemistry (ICC)
(5), (2), (1), (1)
Pubmed 9 references available
Catalog no. ABIN967387
Quantity 0.5 mg
Price
Contact our Customer Service for availability and price in your country.
Options
Shipping to United States (Change)
Add to Basket

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Immunogen Collagenase-dispersed BALB/c lymph node stroma
Clone MECA-79
Isotype IgM, kappa
Specificity Carbohydrate Epitope
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Sodium azide is a reversible inhibitor of oxidative metabolism, therefore, antibody preparations containing this preservative agent must not be used in cell cultures nor injected into animals. Sodium azide may be removed by washing stained cells or plate-bound antibody or dialyzing soluble antibody in sodium azide-free buffer. Since endotoxin may also affect the results of functional studies, we recommend the NA/LE (No Azide/Low Endotoxin) antibody format, if available, for in vitro and in vivo use.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name PNAd
Background The MECA-79 antibody reacts with sulfate-dependent carbohydrate epitopes of peripheral lymph node addressin (PNAd). The MECA-79-reactive antigen is closely associated with the carbohydrate ligands for L-selectin (eg, CD34, GlyCAM-1, MAdCAM-1), which are expressed on high endothelial venules (HEV) in lymphoid tissues and at sites of chronic inflammation. Cross-reactivity with human, sheep, cow, primate, and pig tissues has been observed. MECA-79 antibody inhibits L-selectin-dependent lymphocyte and platelet homing to lymph nodes in vivo, and in vitro adhesion to lymphoid tissue HEV and immobilized PNAd.
Synonyms: CD62L Ligand
Application Notes This antibody has been tested by immunohistochemical staining (IHC) of citrate-pretreated formalin-fixed paraffin-embedded sections (5 - 20 µg/ml) to assure specificity and reactivity. Other reported applications include IHC of acetone-fixed frozen sections, immunoprecipitation, western blot analysis, and in vitro and in vivo adhesion blocking.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
General Berg, Robinson, Warnock et al.: "The human peripheral lymph node vascular addressin is a ligand for LECAM-1, the peripheral lymph node homing receptor." in: The Journal of cell biology, Vol. 114, Issue 2, pp. 343-9, 1991 (PubMed).

Streeter, Rouse, Butcher: "Immunohistologic and functional characterization of a vascular addressin involved in lymphocyte homing into peripheral lymph nodes." in: The Journal of cell biology, Vol. 107, Issue 5, pp. 1853-62, 1988 (PubMed).

Hemmerich, Butcher, Rosen: "Sulfation-dependent recognition of high endothelial venules (HEV)-ligands by L-selectin and MECA 79, and adhesion-blocking monoclonal antibody." in: The Journal of experimental medicine, Vol. 180, Issue 6, pp. 2219-26, 1994 (PubMed).

Puri, Finger, Gaudernack et al.: "Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules." in: The Journal of cell biology, Vol. 131, Issue 1, pp. 261-70, 1995 (PubMed).

Faveeuw, Gagnerault, Lepault: "Expression of homing and adhesion molecules in infiltrated islets of Langerhans and salivary glands of nonobese diabetic mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 12, pp. 5969-78, 1994 (PubMed).

Michie, Streeter, Bolt et al.: "The human peripheral lymph node vascular addressin. An inducible endothelial antigen involved in lymphocyte homing." in: The American journal of pathology, Vol. 143, Issue 6, pp. 1688-98, 1994 (PubMed).

Diacovo, Puri, Warnock et al.: "Platelet-mediated lymphocyte delivery to high endothelial venules." in: Science (New York, N.Y.), Vol. 273, Issue 5272, pp. 252-5, 1996 (PubMed).

Malý, Thall, Petryniak et al.: "The alpha(1,3)fucosyltransferase Fuc-TVII controls leukocyte trafficking through an essential role in L-, E-, and P-selectin ligand biosynthesis." in: Cell, Vol. 86, Issue 4, pp. 643-53, 1996 (PubMed).

Binns, Whyte, Licence et al.: "The role of E-selectin in lymphocyte and polymorphonuclear cell recruitment into cutaneous delayed hypersensitivity reactions in sensitized pigs." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 157, Issue 9, pp. 4094-9, 1996 (PubMed).

Hosts (4), (2)
Reactivities (6), (1)
Applications (5), (2), (1), (1)
Conjugates (1)
Epitopes (2), (2), (1)
back to top