TUBB antibody

Catalog No. ABIN967495

Product Details anti-TUBB Antibody

Target: TUBB (Tubulin, beta (TUBB))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This TUBB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), BioImaging (BI)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. This antibody has been developed and certified for the bioimaging application. However, a routine bioimaging test is not performed on every lot. Researchers are encouraged to titrate the reagent for optimal performance.
3. Triton is a trademark of the Dow Chemical Company.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
5. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Clone: 5H1

Isotype: IgM kappa

Application Details

Application Notes: Bioimaging
1. Seed the cells in appropriate culture medium at ~10,000 cells per well in an 96-well Imaging Plate and culture overnight.
2. Remove the culture medium from the wells, and fix the cells by adding 100 myl of Fixation Buffer to each well. Incubate for 10 minutes at room temperature (RT).
3. Remove the fixative from the wells, and permeabilize the cells using either 90% methanol, or Triton™ X-100: a. Add 100 myl of -20°C 90% methanol to each well and incubate for 5 minutes at RT. OR b. Add 100 myl of 0.1% Triton™ X-100 to each well and incubate for 5 minutes at RT.
4. Remove the permeabilization buffer, and wash the wells twice with 100 myl of 1× PBS.
5. Remove the PBS, and block the cells by adding 100 myl of to each well. Incubate for 30 minutes at RT.
6. Remove the blocking buffer and add 50 myl of the optimally titrated primary antibody (diluted in Stain Buffer) to each well, and incubate for 1 hour at RT.
7. Remove the primary antibody, and wash the wells three times with 100 myl of 1× PBS.
8. Remove the PBS, and add the second step reagent at its optimally titrated concentration in 50 myl to each well, and incubate in the dark for 1 hour at RT.
9. Remove the second step reagent, and wash the wells three times with 100 myl of 1× PBS.
10. Remove the PBS, and counter-stain the nuclei by adding 200 myl per well of 2 myg/ml Hoechst 33342 in 1× PBS to each well at least 15 minutes before imaging.
11. View and analyze the cells on an appropriate imaging instrument.

Comment:

Related Products: ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-TUBB antibody (ABIN967495)

Cho, Johnson: "Primed phosphorylation of tau at Thr231 by glycogen synthase kinase 3beta (GSK3beta) plays a critical role in regulating tau's ability to bind and stabilize microtubules." in: Journal of neurochemistry, Vol. 88, Issue 2, pp. 349-58, (2003) (PubMed).

Helfand, Mikami, Vallee, Goldman: "A requirement for cytoplasmic dynein and dynactin in intermediate filament network assembly and organization." in: The Journal of cell biology, Vol. 157, Issue 5, pp. 795-806, (2002) (PubMed).

Prahlad, Yoon, Moir, Vale, Goldman: "Rapid movements of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament networks." in: The Journal of cell biology, Vol. 143, Issue 1, pp. 159-70, (1998) (PubMed).

Brown, Wood, Cleveland: "The kinesin-like protein CENP-E is kinetochore-associated throughout poleward chromosome segregation during anaphase-A." in: Journal of cell science, Vol. 109 ( Pt 5), Issue 4, pp. 961-9, (1996) (PubMed).

Wang, Loomis, Zinkowski, Binder: "A novel tau transcript in cultured human neuroblastoma cells expressing nuclear tau." in: The Journal of cell biology, Vol. 121, Issue 2, pp. 257-67, (1993) (PubMed).

Target Details for TUBB

Target: TUBB (Tubulin, beta (TUBB))

Alternative Name: beta-Tubulin (TUBB Products)

Synonyms: M40 antibody, OK/SW-cl.56 antibody, TUBB1 antibody, TUBB5 antibody, XLOT antibody, m40 antibody, ok/sw-cl.56 antibody, tubb1 antibody, tubb5 antibody, TUBB2A antibody, TUBB2B antibody, TUBB antibody, 1t antibody, B1t antibody, BETA 56D antibody, CG9277 antibody, DTB2 antibody, Dmbeta1 antibody, Dmel\\CG9277 antibody, T antibody, Tub antibody, Tubulin antibody, beta antibody, beta-Tub antibody, beta-Tub56D antibody, beta-tub antibody, beta-tubulin56D antibody, beta1 antibody, beta1-Tubulin antibody, beta1-tub antibody, beta1Tub antibody, beta1t antibody, beta1tub antibody, beta56D antibody, betaTub antibody, betaTub1 antibody, beta[[1]] tubulin antibody, beta[[1]]-tubulin antibody, betatub(56D) antibody, 143391_i_at antibody, 3t antibody, B3t antibody, BETA 60D antibody, CG3401 antibody, D.m.BETA-60D antibody, DTB3 antibody, Dmbeta3 antibody, Dmel\\CG3401 antibody, Tub60D antibody, beta-Tub60D antibody, beta-Tub6D antibody, beta3 antibody, beta3 TU antibody, beta3-Tub antibody, beta3-tubulin antibody, beta3TUB antibody, beta3Tub antibody, beta3t antibody, beta60C antibody, betaTub3 antibody, betaTub60C antibody, beta[[3]] tubulin antibody, beta[[3]]-Tub antibody, beta[[3]]-tubulin antibody, betatub60D antibody, p50 antibody, p50/tubulin antibody, p53 antibody, p53/tubulin antibody, 2t antibody, B2t antibody, BETA 85D antibody, BETA2 antibody, CG9359 antibody, D.m.BETA-85D antibody, DTB4 antibody, Dmbeta2 antibody, Dmel\\CG9359 antibody, beta(2)Tu antibody, beta(2)Tub antibody, beta-Tub85D antibody, beta-tub85D antibody, beta2 antibody, beta2-tub antibody, beta2t antibody, beta2tub antibody, beta85D antibody, betaTub2 antibody, beta[[2]]-tubulin antibody, ms(3)KK[D] antibody, 4t antibody, B4t antibody, BETA 98B antibody, CG4869 antibody, DTB1 antibody, Dmbeta4 antibody, Dmel\\CG4869 antibody, beta-Tub97EF antibody, beta4 antibody, beta4t antibody, beta97F antibody, betaTub4 antibody, betaTub98BC antibody, betaTub98C antibody, Tub1 antibody, bmtub1 antibody, Tub3 antibody, bmtub3 antibody, Tub2 antibody, bmtub2 antibody, Tub4 antibody, bmtub4 antibody, LOC100101153 antibody, tubulin beta class I antibody, putative cobalt-zinc-cadmium outer membrane resistance protein antibody, Tubulin beta chain antibody, tubulin beta-2A chain antibody, beta-Tubulin at 56D antibody, beta-Tubulin at 60D antibody, beta-Tubulin at 85D antibody, beta-Tubulin at 97EF antibody, beta-tubulin antibody, tubulin beta 6 class V antibody, beta tubulin antibody, tubulin beta class I L homeolog antibody, tubulin, beta 5 class I antibody, TUBB antibody, czcC antibody, tbb-6 antibody, tubb antibody, LOC462399 antibody, betaTub56D antibody, betaTub60D antibody, betaTub85D antibody, betaTub97EF antibody, Tub1 antibody, Tub3 antibody, Tub2 antibody, Tub4 antibody, TVAG_525430 antibody, TVAG_062880 antibody, TVAG_456920 antibody, LOC100101153 antibody, TUBB6 antibody, Tb927.1.2330 antibody, Tb927.1.2350 antibody, Tb927.1.2370 antibody, Tb927.1.2390 antibody, tubb.L antibody, Tubb antibody, LOC693049 antibody, LOC443015 antibody, Tubb5 antibody

Background: Tubulin is a highly conserved protein with a molecular weight of ~50 kD. The self-assembly of tubulin leads to microtubules, hollow cylinders that are one of the major components of the eukaryotic cytoskeleton. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. There are two main classes of tubulin isoforms, alpha- and beta-tubulin, which are usually products of separate genes. Microtubules are made from protofilaments, strings of alternating alpha- and beta-tubulin spaced 4 nm apart and pointing in the same direction. Tubulin can be posttranslationally modified in several ways, including phosphorylation, acetylation, glutamylation, and detyrosination. For example, microtubules that turn over slowly tend to be acetylated and detyrosinated.
The 5H1 monoclonal antibody reacts with beta-tubulin. It does not cross-react with alpha-tubulin.

Molecular Weight: 50 kDa

Pathways: Microtubule Dynamics, M Phase