Serotonin Receptor 5-HT 2BR antibody

Antigen: Serotonin Receptor 5-HT 2BR

Clonality: Monoclonal (A72-1)

Host: Mouse

Reactivity: Human

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Catalog no.: ABIN967504

Additional Information

Immunogen: Human 5HT[2B]R fusion protein

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: A72-1

Description: Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor, plays an important role in regulating diverse biological processes in the central and peripheral nervous systems, the cardiovascular system and the gastrointestinal systems. The different functions of serotonin are thought to be mediated through specific receptors. Physiological, biochemical, and pharmacological studies have defined at least fourteen serotonin receptors. The fourteen receptors are grouped in seven subtypes according to operational, structural, and transductional properties. The 5-HT2 receptors (5 -HT[2A]R, 5-HT[2B]R, an d 5-HT[2C]R) may be considered as prototypes for the study of serotonin receptors . Their functions are mediated by the second messenger inositol polyphosphate in a G-protein coupled pathway. They are expressed in diverse tissues including brain, vascular smooth muscles and platelets. The 5-HT[2A]R-mRNAs have been found in only a few areas of the brain, including the frontal cortex, piriform cortex, ventro-caudal part of CA3, motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal cord. The 5-HT[2C]R mRNA has been found at high levels in many parts of the brain including retrosplenial piriform and entorhinal cortex, anterior olfactory nucleus, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. The 5-HT[2B]R was cloned in stomach fundus, where it is abundantly expressed.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 45-55 kDa

Application Details

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Restrictions: For Research Use only

Images

Immunohistochemical staining of neurons in rat brain (hippocampus) (first panel). A zinc-fixed, paraffin-embedded section of a rat brain was stained with 1.25 µg/mL of the Purified Mouse Anti-Serontonin Receptor 5-HT2BR antibody. Detection was performed using a Biotin Goat Anti-Mouse Ig (MN 550337) secondary and Streptavidin-HRP (MN 550946) followed by development with a DAB chromogen (MN 550880). Arrows are indicative of 5-HT2BR immunoreactivity.

Western blot analysis for 5-HT2BR (second panel). A rat whole brain lysate was probed with 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the Mouse Anti-Serontonin Receptor 5-HT2BR antibody. The serotonin receptor 5-HT2BR is observable at 45-55 kDa with another unidentified band at 60-70 kDa.

Publications

Product: Foguet, Hoyer, Pardo et al.: "Cloning and functional characterization of the rat stomach fundus serotonin receptor." in: The EMBO journal, Vol. 11, Issue 9, pp. 3481-7, 1992 (PubMed).

Saudou, Hen: "5-Hydroxytryptamine receptor subtypes in vertebrates and invertebrates." in: Neurochemistry international, Vol. 25, Issue 6, pp. 503-32, 1995 (PubMed).

Pompeiano, Palacios, Mengod: "Distribution of the serotonin 5-HT2 receptor family mRNAs: comparison between 5-HT2A and 5-HT2C receptors." in: Brain research. Molecular brain research, Vol. 23, Issue 1-2, pp. 163-78, 1994 (PubMed).

Humphrey, Hartig, Hoyer: "A proposed new nomenclature for 5-HT receptors." in: Trends in pharmacological sciences, Vol. 14, Issue 6, pp. 233-6, 1993 (PubMed).

Duxon, Flanigan, Reavley et al.: "Evidence for expression of the 5-hydroxytryptamine-2B receptor protein in the rat central nervous system." in: Neuroscience, Vol. 76, Issue 2, pp. 323-9, 1997 (PubMed).