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Serotonin Receptor 5-HT 2BR antibody

Details for Product No. ABIN967504
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Antigen
Reactivity
Human
Host
Mouse
Clonality (Clone)
Monoclonal ()
Application
Immunohistochemistry (IHC), Western Blotting (WB)
Pubmed 5 references available
Quantity 0.1 mg
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Catalog No. ABIN967504
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Immunogen Human 5HT[2B]R fusion protein
Clone A72-1
Isotype IgG1
Cross-Reactivity Rat (Rattus)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Background Serotonin (5-hydroxytryptamine, 5-HT), originally discovered as a serum factor, plays an important role in regulating diverse biological processes in the central and peripheral nervous systems, the cardiovascular system and the gastrointestinal systems. The different functions of serotonin are thought to be mediated through specific receptors. Physiological, biochemical, and pharmacological studies have defined at least fourteen serotonin receptors. The fourteen receptors are grouped in seven subtypes according to operational, structural, and transductional properties. The 5-HT2 receptors (5 -HT[2A]R, 5-HT[2B]R, an d 5-HT[2C]R) may be considered as prototypes for the study of serotonin receptors . Their functions are mediated by the second messenger inositol polyphosphate in a G-protein coupled pathway. They are expressed in diverse tissues including brain, vascular smooth muscles and platelets. The 5-HT[2A]R-mRNAs have been found in only a few areas of the brain, including the frontal cortex, piriform cortex, ventro-caudal part of CA3, motor cranial nerve nuclei in the brainstem, and the ventral horn of the spinal cord. The 5-HT[2C]R mRNA has been found at high levels in many parts of the brain including retrosplenial piriform and entorhinal cortex, anterior olfactory nucleus, substantia nigra pars compacta, several brainstem nuclei and the whole grey matter of the spinal cord. The 5-HT[2B]R was cloned in stomach fundus, where it is abundantly expressed.
Molecular Weight 45-55 kDa
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Supplier Images
anti-Serotonin Receptor 5-HT 2BR antibody Immunohistochemical staining of neurons in rat brain (hippocampus) (first panel). A zinc-fixed, paraffin-embedded section of a rat brain was stained with 1.25 µg/mL of the Purified Mouse Anti-Serontonin Receptor 5-HT2BR antibody. Detection was performed using a Biotin Goat Anti-Mouse Ig (MN 550337) secondary and Streptavidin-HRP (MN 550946) followed by development with a DAB chromogen (MN 550880). Arrows are indicative of 5-HT2BR immunoreactivity.
anti-Serotonin Receptor 5-HT 2BR antibody (2) Western blot analysis for 5-HT2BR (second panel). A rat whole brain lysate was probed with 2 µg/mL (lane 1), 1 µg/mL (lane 2) and 0.5 µg/mL (lane 3) of the Mouse Anti-Serontonin Receptor 5-HT2BR antibody. The serotonin receptor 5-HT2BR is observable at 45-55 kDa with another unidentified band at 60-70 kDa.
Product cited in: Foguet, Hoyer, Pardo et al.: "Cloning and functional characterization of the rat stomach fundus serotonin receptor." in: The EMBO journal, Vol. 11, Issue 9, pp. 3481-7, 1992 (PubMed).

Saudou, Hen: "5-Hydroxytryptamine receptor subtypes in vertebrates and invertebrates." in: Neurochemistry international, Vol. 25, Issue 6, pp. 503-32, 1995 (PubMed).

Pompeiano, Palacios, Mengod: "Distribution of the serotonin 5-HT2 receptor family mRNAs: comparison between 5-HT2A and 5-HT2C receptors." in: Brain research. Molecular brain research, Vol. 23, Issue 1-2, pp. 163-78, 1994 (PubMed).

Humphrey, Hartig, Hoyer: "A proposed new nomenclature for 5-HT receptors." in: Trends in pharmacological sciences, Vol. 14, Issue 6, pp. 233-6, 1993 (PubMed).

Duxon, Flanigan, Reavley et al.: "Evidence for expression of the 5-hydroxytryptamine-2B receptor protein in the rat central nervous system." in: Neuroscience, Vol. 76, Issue 2, pp. 323-9, 1997 (PubMed).

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