Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody

Details for Product No. ABIN967559
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Synonyms NF-ATC, NFAT2, NFATc, NF-ATc, Nfatcb, AI449492, AV076380, 2210017P03Rik, NF-ATc1, NFATmac, NFAT, nf-atc, nfat2, nfatc, si:ch211-198c22.2, NFATC1
(74), (28), (19), (2), (1)
(55), (21), (1)
Clonality (Clone)
Monoclonal ()
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Western Blotting (WB), Gel Shift (GS), Immunofluorescence (IF), Immunoprecipitation (IP)
(60), (28), (15), (10), (9), (6), (3), (2), (2), (2), (1), (1), (1)
Pubmed 5 references available
Catalog no. ABIN967559
Quantity 0.1 mg
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Clone 7A6
Isotype IgG1
Cross-Reactivity Mouse (Murine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Purity Purified
Alternative Name NF-ATc1
Background The NF-AT family of transcription factors are regulators of early immune response genes in T cells following their activation by CD40L, FasL and nterleukins including IL-2, -3, -4 and -5. NF-AT proteins function in both signal transduction and transcription control. The DNA binding, active NF-AT complex contains a cytoplasmic, Ca2+/calcineurin dependent, cyclosporin sensitive subunit, designated NF-ATc, which is present in the cytoplasm of resting cells. Upon cell activation, NF-ATc is dephosphorylated and thus activated by calcineurin, resulting in rapid translocation of NF-ATc to the nucleus. The active NF-AT complex also contains a nuclear component, NF-ATn. In vitro, NF-AT cooperates with the mitogenic transcription factor AP-1 to induce multiple cytokine genes. NF-ATc is encoded by four genes: NF-ATc1 (originally named NF-ATc), NF-ATc2 (originally named NF-ATp), NF-ATc3 (NF-AT3) and NF- ATc4 (NF-AT4). These proteins are differentially expressed in tissues, suggesting that each may activate distinct sets of genes. NF-ATc1 is expressed in lymphoid organs and is upregulated in activated T cell and thymocytes. NF-ATc1 is required for the development of T cells and has been shown to contribute to the production of IL-2 and the development of Th2 responses. 7A6 recognizes human and mouse NF-ATc1. The antibody recognizes a region between amino acids 197 to 304 of human NF-ATc1, which lies outside the region of homology between NF-ATc1 and NF-ATc2.3 Please note that in the original publication for the antibody (Northrop et al. 1994), the clone name 7A6 is not specifically identified.
Molecular Weight 100-120 kDa
Application Notes 7A6 may be used for western blot analysis of NF-ATc1 (1-2 µg/ml). Note that while the predicted M W of NF-ATc is ~100 kDa, the observed M W may range between 100 to 120 kDa due to post-translational modification of NF-ATc. Other applications include immunoprecipitation (2 µg/ml), mobility shift assays (EMSA) and immunofluorescence microscopy of paraformaldehyde-fixed, tissue cultured cells. Jurkat human T cells (ATCC TIB-152) are suggested as a positive control for expression of NF-ATc.

Related Products: ABIN967389, ABIN968537

Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Storage 4 °C
Supplier Images
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody Western blot analysis of NFATc1. Lysate from Jurkat T cells was probed with anti-NF-ATc1 (ABIN967559). The antibody identifies NF-ATc1 isoforms at a range of molecular weights from ~100 to 120 kDa.
anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody (2) anti-Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 1 (NFATC1) antibody (Image 2)
Product cited in: Crabtree: "Contingent genetic regulatory events in T lymphocyte activation." in: Science (New York, N.Y.), Vol. 243, Issue 4889, pp. 355-61, 1989 (PubMed).

Northrop, Ho, Chen et al.: "NF-AT components define a family of transcription factors targeted in T-cell activation." in: Nature, Vol. 369, Issue 6480, pp. 497-502, 1994 (PubMed).

Timmerman, Clipstone, Ho et al.: "Rapid shuttling of NF-AT in discrimination of Ca2+ signals and immunosuppression." in: Nature, Vol. 383, Issue 6603, pp. 837-40, 1996 (PubMed).

Timmerman, Healy, Ho et al.: "Redundant expression but selective utilization of nuclear factor of activated T cells family members." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 6, pp. 2735-40, 1997 (PubMed).

Yoshida, Nishina, Takimoto et al.: "The transcription factor NF-ATc1 regulates lymphocyte proliferation and Th2 cytokine production." in: Immunity, Vol. 8, Issue 1, pp. 115-24, 1998 (PubMed).

Hosts (55), (21), (1)
Reactivities (74), (28), (19), (2), (1)
Applications (60), (28), (15), (10), (9), (6), (3), (2), (2), (2), (1), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Epitopes (11), (6), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
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