Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6) (pTyr641) antibody

Antigen: Signal Transducer and Activator of Transcription 6, Interleukin-4 Induced (STAT6)

Synonyms: STAT6B, STAT6C, D12S1644, IL-4-STAT

Binding Site: pTyr641

Clonality: Monoclonal (J71-773-58-11)

Host: Mouse

Reactivity: Mouse (Murine)

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Catalog no.: ABIN967582

Additional Information

Alternative name: Stat6

Immunogen: Phosphorylated Mouse STAT6 (Y641) Peptide

Format: Liquid

Isotype: IgG1, kappa

Clone: J71-773-58-11

Description: STATs (signal transducers and activators of transcription) are critical mediators of the biologic activity of cytokines including Interleukins (IL) 2-5, IL-7, IL-15, GM-CSF, erythropoietin and growth hormone. Ligand-receptor interaction leads to activation of constitutively associated JAK family kinases and subsequent recruitment/activation of STATs by tyrosine phosphorylation. Active STATs then move to the nucleus to promote transcription of cytokine-inducible genes. Seven STAT proteins have been cloned, each of which is differentially expressed and/or activated in a cytokine-specific and cell type-specific manner. Stat6 plays an important role in signaling pathways that lead to the differentiation of T helper type 2 (Th2) cells from uncommitted CD4 T cell precursors. Moreover, IL-4, secreted by activated T lymphocytes, basophils, and mast cells, induces specific gene expression via the induction of tyrosine phosphorylation of Stat6 at tyrosine 641 (Y641). The SH3:SH2 domain of Stat6 associates with tyrosine-phosphorylated IL-4 receptor and the proximal Jak kinase phosphorylates Stat6 at Y641 on the C-terminal side of the SH2 domain. Stat6 is then released from the receptor, dimerizes, and is thought to contact the basal transcription machinery by binding to p300/CBP. While Stat6 is widely expressed in human tissues, it exhibits elevated expression in peripheral blood lymphocytes, colon, intestine, ovary, prostate, thymus, spleen, kidney, liver, lung, and placenta.
The J71-773.58.11 antibody recognizes mouse Stat6 phosphorylated at Y641.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Please refer to us for technical protocols.

Molecular Weight: 97 kDa

Comments:

Related Products: ABIN967389, ABIN968593

Application Details

Application Notes: For western blotting of human samples, Purified Mouse Anti-Human Stat6 (pY641) mAb (clone 18) (ABIN968593) is recommended.

Concentration: 0.5 mg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at 4°C.

Research Area: Signaling, Receptors, Inflammation

Restrictions: For Research Use only

Images

Western blot analysis of mouse Stat6 (pY641). Lysates from control (First Panel) and mouse IL-4 treated (Second Panel) mouse 2E9 T hybridoma were probed with purified mouse anti-Stat6 (pY641) mAb (clone J71-773.58.11) at concentrations of 2.0 (lanes 1), 1.0 (lanes 2), and 0.5 (lanes 3) µg/ml. Stat6 (pY641) is identified as a band of 97 kDa in the treated lysate.

Publications

Product: Quelle, Shimoda, Thierfelder et al.: "Cloning of murine Stat6 and human Stat6, Stat proteins that are tyrosine phosphorylated in responses to IL-4 and IL-3 but are not required for mitogenesis." in: Molecular and cellular biology, Vol. 15, Issue 6, pp. 3336-43, 1995 (PubMed).

Hou, Schindler, Henzel et al.: "An interleukin-4-induced transcription factor: IL-4 Stat." in: Science (New York, N.Y.), Vol. 265, Issue 5179, pp. 1701-6, 1994 (PubMed).

Heim: "The Jak-STAT pathway: specific signal transduction from the cell membrane to the nucleus." in: European journal of clinical investigation, Vol. 26, Issue 1, pp. 1-12, 1996 (PubMed).

Mikita, Campbell, Wu et al.: "Requirements for interleukin-4-induced gene expression and functional characterization of Stat6." in: Molecular and cellular biology, Vol. 16, Issue 10, pp. 5811-20, 1996 (PubMed).

Dent, Hu-Li, Paul et al.: "T helper type 2 inflammatory disease in the absence of interleukin 4 and transcription factor STAT6." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 23, pp. 13823-8, 1998 (PubMed).

Bromberg, Darnell: "The role of STATs in transcriptional control and their impact on cellular function." in: Oncogene, Vol. 19, Issue 21, pp. 2468-73, 2000 (PubMed).

Hale, Krutzik, Samra et al.: "Stage dependent aberrant regulation of cytokine-STAT signaling in murine systemic lupus erythematosus." in: PLoS ONE, Vol. 4, Issue 8, pp. e6756, 2009 (PubMed).