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Integrin beta 1 (ITGB1) antibody

Antigen

Integrin beta 1 (ITGB1)
Synonyms CD29, FNRB, MDF2, VLAB, GPIIA, MSK12, VLA-BETA, Fnrb, gpIIa, AA409975, AA960159, 4633401G24Rik, ITGB1, MGC137337, itgb1-B
Clonality Monoclonal (KMI6)
Host
Reactivity
Alternatives

Mouse (Murine)
Conjugate
Alternatives Un-conjugated
Application
Alternatives Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP), Western Blotting (WB)
4 references available
Catalog no. ABIN967623
Quantity 0.1 mg  (0.5 mg/ml)
Price Product not available in this region.
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Application Details Publications Alternatives back to top

Alternative name CD29
Immunogen C57BL/6 x DBA/2 F1 mouse bone-marrow stromal cell clone BMS2
Format Liquid
Isotype IgG2a, kappa
Clone KMI6
Description The KMI6 antibody reacts with the 130-kDa integrin ß1 chain (CD29). CD29 is expressed on the cell surface as a heterodimer with one of the distinct integrin achains. With a1 through a6 (CD49a through CD49f), it forms the VLA-1 through VLA-6 complexes, respectively, and with av (CD51), it forms avß1 integrin. It also associates with the integrin a7, a8, and a9 chains in non-lymphoid tissues. As a result, CD29 has a broad tissue distribution, including leukocytes, endothelia, smooth muscle, epithelia, and oocytes. The epitope recognized by the KMI6 mAb can be detected by immunoblot in unreduced extracts from a variety of cells and tissues having a number of different ß1-integrin heterodimer combinations. However, the epitope is not well expressed on the surface of the majority of hematopoietic cells. It is detected on the surface of fetal liver cells, some bone marrow stromal cells, and some cultured hematopoietic cell lines, including P815 and PA6. Surface expression of the CD29 epitope recognized by mAb KMI6 on thymocytes and T lymphocytes appears to be developmentally regulated, and KMI6 antibody enhances the adhesion of KMI6 thymocytes to fibronectin and laminin.
Synonyms: Integrin beta1 chain
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Application Details
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Application Notes Please note that the epitope is lost upon sulfhydryl reduction of the antigen. For IHC, we recommend the use of purified anti-mouse CD29 mAb 9EG7 mAb in our special formulation for immunohistochemistry.
Concentration 0.5 mg/ml
Purity Purified
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Buffer Aqueous buffered solution.
Preservative 0.09% Sodium azide.
Storage Store undiluted at 4° C.
Research Area CD Antigens, Surface Receptors of Immune Cells
Restrictions For Research Use only

Publications
Application Details Alternatives back to top

Jacobsen, Miyake, Kincade et al.: "Highly restricted expression of a stromal cell determinant in mouse bone marrow in vivo." in: The Journal of experimental medicine, Vol. 176, Issue 4, pp. 927-35, 1992 (PubMed).

Springer: "Adhesion receptors of the immune system." in: Nature, Vol. 346, Issue 6283, pp. 425-34, 1990 (PubMed).

Wu, Miyake, Medina et al.: "Recognition of murine integrin beta 1 by a rat anti-stromal cell monoclonal antibody." in: Hybridoma, Vol. 13, Issue 5, pp. 409-16, 1995 (PubMed).

Wadsworth, Chang, Hong et al.: "Expression of a novel integrin beta 1 chain epitope and anti-beta 1 antibody-mediated enhancement of fibronectin binding are dependent on the stage of T cell differentiation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 154, Issue 5, pp. 2125-33, 1995 (PubMed).

Alternatives
Application Details Publications back to top

Alternatives for antigen "Integrin beta 1 (ITGB1)", type "Antibodies"
Hosts Mouse (137), Rabbit (63), Hamster (36), Rat (5), Chicken (2)
Reactivities Human (201), Mouse (Murine) (80), Rat (Rattus) (50), Dog (Canine) (38), Pig (Porcine) (37), Chicken (20), Cow (Bovine) (19), Horse (Equine) (18), Sheep (Ovine) (18), Monkey (4), Cat (Feline) (1), Rabbit (1)
Applications Flow Cytometry (FACS) (158), Western Blotting (WB) (108), ELISA (56), Immunoprecipitation (IP) (55), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (44), Immunofluorescence (IF) (31), Immunohistochemistry (Fixed) (IHC (fx)) (22), Immunohistochemistry (IHC) (18), Immunohistochemistry (Frozen Sections) (IHC (fro)) (12), Immunocytochemistry (ICC) (11), Functional Studies (Func) (7), Enzyme Immunoassay (EIA) (4), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Dot Blot (DB) (1), Immunoelectron Microscopy (IEM) (1), Immunohistochemistry (Paraformaldehyde) (IHC (pfa)) (1)
Conjugates FITC (23), Biotin (21), PE (19), APC (9), RPE (5), Cy7,PE (2), PE-Dyomics 647 (2), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), HRP (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE,DY647 (1)
Epitopes Extracellular Domain (6), pTyr795,C-Term (6), C-Term (4), N-Term (3), AA 34-141 (2), pTyr783 (2), AA 21-140 (1), C-Term,AA 758-785 (1), Cytoplasmic Domain (1), pThr788 (1), pThr789 (1)