Platelet-Derived Growth Factor Receptor, alpha Polypeptide (PDGFRA) antibody

Details for Product No. ABIN967626
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Antigen
Synonyms AI115593, CD140a, Pdgfr-2, APDGFR, PDGFACE, CD140A, PDGFR-2, PDGFR2, RHEPDGFRA, PDGFR, PDGFR-A
Reactivity
Mouse (Murine)
(294), (173), (143), (84), (72), (37), (36), (1)
Host
Rat
(271), (31), (19), (2), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(21), (16), (14), (13), (12), (7), (7), (7), (7), (7), (7), (7), (7), (7), (1)
Application
Flow Cytometry (FACS), ELISA, Functional Studies (Func), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(171), (130), (91), (70), (68), (35), (23), (18), (12), (6), (3), (3), (2), (2), (2)
Pubmed 3 references available
Quantity 0.1 mg
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Catalog No. ABIN967626
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Immunogen Mouse PDGF Receptor alpha chain
Clone APA5
Isotype IgG2a, kappa
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name CD140a
Background The APA5 antibody reacts with Platelet-Derived Growth Factor (PDGF) Receptor alpha chain (CD140a, PDGFR-a), a receptor tyrosine kinase which is widely expressed on cells of mesenchymal origin in the embryo and adult and on several other cell types during embryonic development, but not on hematopoietic cells. PDGFR-a binds to PDGF A and B chains, in contrast to PDGFR-b, which binds only to the PDGF B chain. Biologically active PDGF is a disulphide-linked dimer, forming the AA, AB, and BB isoforms. Binding to PDGF Receptor induces the formation of receptor dimers (aa, ab, or bb), autophosphorylation, and internalization. The mAb APA5 has been demonstrated to block binding of PDGF-AA to PDGFR-a-expressing cells in vitro and to block some PDGF-mediated developmental events in vivo.
Synonyms: PDGF Receptor alpha chain
Research Area CD Antigens, Surface Receptors of Immune Cells
Application Notes This antibody has been tested by immunofluorescent staining (Less or equal than 1 µg/million cells) with flow cytometric analysis and immunohistochemical staining of acetone-fixed frozen sections (0.5 - 2.5 µg/ml) to assure specificity and reactivity. Other reported applications include western blot analysis, ELISA, immunohistochemical staining of paraffin-embedded sections, inhibition of ligand binding, and in vivo blocking of PDGF action.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/ml
Buffer Aqueous buffered solution.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Product cited in: Heldin: "Structural and functional studies on platelet-derived growth factor." in: The EMBO journal, Vol. 11, Issue 12, pp. 4251-9, 1992 (PubMed).

Takakura, Yoshida, Kunisada et al.: "Involvement of platelet-derived growth factor receptor-alpha in hair canal formation." in: The Journal of investigative dermatology, Vol. 107, Issue 5, pp. 770-7, 1996 (PubMed).

Fruttiger, Calver, Krüger et al.: "PDGF mediates a neuron-astrocyte interaction in the developing retina." in: Neuron, Vol. 17, Issue 6, pp. 1117-31, 1997 (PubMed).

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