TNFRSF4 antibody

Catalog No. ABIN967651

This Rat Monoclonal antibody specifically detects TNFRSF4 in WB, FACS and IHC (fro). It exhibits reactivity toward Mouse. It has been mentioned in 8+ publications

Quick Overview for TNFRSF4 antibody (ABIN967651)

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This TNFRSF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-86

Product Details anti-TNFRSF4 Antibody

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Recombinant Mouse OX-40 3/4 CD4 Chimeric Protein

Isotype: IgG1 kappa

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4° C.

References for anti-TNFRSF4 antibody (ABIN967651)

Pippig, Peña-Rossi, Long, Godfrey, Fowell, Reiner, Birkeland, Locksley, Barclay, Killeen: "Robust B cell immunity but impaired T cell proliferation in the absence of CD134 (OX40)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 12, pp. 6520-9, (2000) (PubMed).

Akiba, Oshima, Takeda, Atsuta, Nakano, Nakajima, Nohara, Yagita, Okumura: "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 12, pp. 7058-66, (1999) (PubMed).

Higgins, McDonald, Whittle, Crockett, Shields, MacDonald et al.: "Regulation of T cell activation in vitro and in vivo by targeting the OX40-OX40 ligand interaction: amelioration of ongoing inflammatory bowel disease with an OX40-IgG fusion protein, but not with an ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 1, pp. 486-93, (1999) (PubMed).

Weinberg, Vella, Croft: "OX-40: life beyond the effector T cell stage." in: Seminars in immunology, Vol. 10, Issue 6, pp. 471-80, (1999) (PubMed).

Weinberg, Wegmann, Funatake, Whitham: "Blocking OX-40/OX-40 ligand interaction in vitro and in vivo leads to decreased T cell function and amelioration of experimental allergic encephalomyelitis." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 3, pp. 1818-26, (1999) (PubMed).

al-Shamkhani, Birkeland, Puklavec, Brown, James, Barclay: "OX40 is differentially expressed on activated rat and mouse T cells and is the sole receptor for the OX40 ligand." in: European journal of immunology, Vol. 26, Issue 8, pp. 1695-9, (1996) (PubMed).

Stüber, Neurath, Calderhead, Fell, Strober: "Cross-linking of OX40 ligand, a member of the TNF/NGF cytokine family, induces proliferation and differentiation in murine splenic B cells." in: Immunity, Vol. 2, Issue 5, pp. 507-21, (1995) (PubMed).

Baum, Gayle, Ramsdell, Srinivasan, Sorensen, Watson, Seldin, Baker, Sutherland, Clifford: "Molecular characterization of murine and human OX40/OX40 ligand systems: identification of a human OX40 ligand as the HTLV-1-regulated protein gp34." in: The EMBO journal, Vol. 13, Issue 17, pp. 3992-4001, (1994) (PubMed).

Target Details for TNFRSF4

Target: TNFRSF4 (Tumor Necrosis Factor Receptor Superfamily, Member 4 (TNFRSF4))

Alternative Name: CD134

Background: The OX-86 mAb reacts with the OX-40 antigen (CD134), also known as OX-40 receptor, which is a 50-kDa type-I membrane glycoprotein that belongs to the NGFR/TNFR superfamily. Mouse CD134 is expressed on activated CD4+ and CD8+ T lymphocytes and has been shown to be the sole receptor for the OX-40 Ligand (OX-40L). In the brains of mice with actively induced experimental allergic encephalomyelitis, the expression of CD134 on CD4+ T lymphocytes correlates with disease progression. The OX-40/OX-40L system supplies a costimulatory signal for T-cell proliferation and B-cell proliferation and differentiation. In addition, OX-40 antigen provides a costimulatory signal that induces T cells to proliferate in a CD28-independent manner. In the intact animal, CD134 does not appear to be essential for many T-cell responses, but it seems to play a major role in the pathogenesis of some autoimmune diseases. The OX-86 mAb stains both CD4+ and CD8+ activated T cells, and this expression pattern has been confirmed using OX-40L-Ig fusion protein. CD134 was also detected, using OX-86 mAb, on B cells after stimulation with anti-IgM plus anti-CD40 mAb HM40-3. OX-86 mAb does not block binding of OX-40L to OX-40, and it stimulates T-cell proliferation mildly.
Synonyms: OX-40 Antigen

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints