Western blot analysis of TAZ in transformed human epithelioid carcinoma. HeLa cell lysate (ABIN968535) was probed with Purified Mouse anti-TAZ monoclonal antibody at concentrations of 0.5, 0.25, and 0.125 µg/ml (lanes 1, 2, and 3, respectively). TAZ is identified as a band of 49 kDa.
Immunofluorescent staining of human cell lines. HeLa cells (ATCC CCL-2) were seeded in two 96-well imaging plates at ~10,000 cells per well. After overnight incubation, the cells were fixed, permeabilized with Saponin, and stained with Purified Mouse anti-TAZ (pseudocolored green) according to the Recommended Assay Procedure. The second-step reagent was Alexa Fluor® 555 goat anti-mouse Ig (Invitrogen). The left image shows TAZ alone, and the right image shows TAZ merged with Hoechst staining. Images were captured on a BD Pathway™ 435 bioimager using a 20x objective and merged using BD AttoVision™ software. Other permeabilization methods, cold methanol and Triton™ X-100, did not work well.