FGF2 antibody (AA 1-155)

Catalog No. ABIN967725

This anti-FGF2 antibody is a Mouse Monoclonal antibody detecting FGF2 in WB, IHC, IP and IF. Suitable for Human, Mouse, Rat, Dog and Chicken. This Primary Antibody has been cited in 5+ publications.

Quick Overview for FGF2 antibody (AA 1-155) (ABIN967725)

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This FGF2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 6-basic FGF

Product Details anti-FGF2 Antibody

Binding Specificity: AA 1-155

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human bFGF aa. 1-155

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-FGF2 antibody (ABIN967725)

Yaccoby, Johnson, Mahaffey, Wezeman, Barlogie, Epstein: "Antimyeloma efficacy of thalidomide in the SCID-hu model." in: Blood, Vol. 100, Issue 12, pp. 4162-8, (2002) (PubMed).

Peng, Moffett, Myers, Fang, Stachowiak, Maher, Kratz, Hines, Fluharty, Mizukoshi, Bloom, Stachowiak: "Novel nuclear signaling pathway mediates activation of fibroblast growth factor-2 gene by type 1 and type 2 angiotensin II receptors." in: Molecular biology of the cell, Vol. 12, Issue 2, pp. 449-62, (2001) (PubMed).

Estival, Monzat, Miquel, Gaubert, Hollande, Korc, Vaysse, Clemente: "Differential regulation of fibroblast growth factor (FGF) receptor-1 mRNA and protein by two molecular forms of basic FGF. Modulation of FGFR-1 mRNA stability." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5663-70, (1996) (PubMed).

Fox, Shanley: "Antisense inhibition of basic fibroblast growth factor induces apoptosis in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 271, Issue 21, pp. 12578-84, (1996) (PubMed).

Stachowiak, Moffett, Joy, Puchacz, Florkiewicz, Stachowiak: "Regulation of bFGF gene expression and subcellular distribution of bFGF protein in adrenal medullary cells." in: The Journal of cell biology, Vol. 127, Issue 1, pp. 203-23, (1994) (PubMed).

Target Details for FGF2

Target: FGF2 (Fibroblast Growth Factor 2 (Basic) (FGF2))

Alternative Name: basic FGF

Background: Basic Fibroblast Growth Factor (bFGF) is a family member of the cell-differentiating and growth promoting factors. Basic FGF exists as multiple isoforms ranging from 18-24kDa that are derived from a single mRNA species under unique translational conditions. The 18kDa isoform is primarily cytosolic, but is also secreted and may form reservoirs of bFGF in the extracellular matrix. In contrast, the larger isoforms are predominantly nuclear. At the cellular level, bFGF is a potent mitogen and promotes cell survival by inhibiting apoptosis. At the tissue level, it is involved in wound repair and induces angiogenesis. In addition, bFGF is a significant target in cancer research because it is over-expressed in some cancers and may enhance a tumor's metastatic potential.

Molecular Weight: 18-24 kDa

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, C21-Steroid Hormone Metabolic Process, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Protein targeting to Nucleus, S100 Proteins