basic FGF (AA 1-155) antibody

Antigen: basic FGF

Binding Site: AA 1-155

Clonality: Monoclonal (6)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967725

Additional Information

Immunogen: Human bFGF

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Dog (Canine), Chicken

Format: Liquid

Isotype: IgG1

Clone: 6

Description: Basic Fibroblast Growth Factor (bFGF) is a family member of the cell-differentiating and growth promoting factors. Basic FGF exists as multiple isoforms ranging from 18-24kDa that are derived from a single mRNA species under unique translational conditions. The 18kDa isoform is primarily cytosolic, but is also secreted and may form reservoirs of bFGF in the extracellular matrix. In contrast, the larger isoforms are predominantly nuclear. At the cellular level, bFGF is a potent mitogen and promotes cell survival by inhibiting apoptosis. At the tissue level, it is involved in wound repair and induces angiogenesis. In addition, bFGF is a significant target in cancer research because it is over-expressed in some cancers and may enhance a tumor's metastatic potential.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 18-24 kDa

Comments:

Related Products: ABIN968545, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis for basic FGF on a HeLa cell lysate (Human cervical epitheloid carcinoma, ATCC CCL-2). Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-basic FGF antibody.

Immnuofluroescence staining of human fibroblasts.

Publications

Product: Stachowiak, Moffett, Joy et al.: "Regulation of bFGF gene expression and subcellular distribution of bFGF protein in adrenal medullary cells." in: The Journal of cell biology, Vol. 127, Issue 1, pp. 203-23, 1994 (PubMed).

Estival, Monzat, Miquel et al.: "Differential regulation of fibroblast growth factor (FGF) receptor-1 mRNA and protein by two molecular forms of basic FGF. Modulation of FGFR-1 mRNA stability." in: The Journal of biological chemistry, Vol. 271, Issue 10, pp. 5663-70, 1996 (PubMed).

Fox, Shanley: "Antisense inhibition of basic fibroblast growth factor induces apoptosis in vascular smooth muscle cells." in: The Journal of biological chemistry, Vol. 271, Issue 21, pp. 12578-84, 1996 (PubMed).

Peng, Moffett, Myers et al.: "Novel nuclear signaling pathway mediates activation of fibroblast growth factor-2 gene by type 1 and type 2 angiotensin II receptors." in: Molecular biology of the cell, Vol. 12, Issue 2, pp. 449-62, 2001 (PubMed).

Yaccoby, Johnson, Mahaffey et al.: "Antimyeloma efficacy of thalidomide in the SCID-hu model." in: Blood, Vol. 100, Issue 12, pp. 4162-8, 2002 (PubMed).