Mitogen-Activated Protein Kinase 1 (MAPK1) (AA 219-358) antibody
Alternatives Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)
|5 references available|
|Price||Product not available in this region.|
|Cross-Reactivity||Human, Dog (Canine), Mouse (Murine), Chicken, Frog|
|Description||The family of serine/threonine kinases known as ERKs (extracellular signal regulated kinases) or MAPKs (mitogen-activated protein kinases) are activated after cell stimulation by a wide variety of hormones and growth factors. Cell stimulation induces a signaling cascade that leads to phosphorylation of MEK (MAPK/ERK kinase) which, in turn, activates ERK via tyrosine and threonine phosphorylation. Structural analysis of ERK2 indicates that phosphorylation induces a conformational change that exposes the active site for substrate binding. Myriad proteins represent the downstream effectors for the active ERK and implicate it in the control of cell proliferation and differentiation, as well as regulation of the cytoskeleton. Activation of ERK is normally transient and cells possess dual specificity phosphatases that are responsible for its down-regulation. Furthermore, multiple studies have shown that elevated ERK activity is associated with some cancers. ERK2 is the 42kDa member of the ERK family and is highly homologous to ERK1.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||42 kDa|
Related Products: ABIN967389
|Synonyms||ERK, p38, p40, p41, ERK2, ERT1, MAPK2, PRKM1, PRKM2, P42MAPK, p41mapk, ERK3, PRKM6, p97MAPK, HsT17250, DKFZp686F03189, Erk2, Prkm1, C78273, p42mapk, AA407128, AU018647, 9030612K14Rik, zERK2, MAPK1, Xp42, erk, erk2, mapk, mpk1, xp42, mapk1a, MGC68934, ert1, mapk2, prkm1, prkm2, F14N23.9, F14N23_9, MITOGEN-ACTIVATED PROTEIN KINASE, MITOGEN-ACTIVATED PROTEIN KINASE 1, MPK1|
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Research Area||Stem Cells, Signaling, Inflammation|
|Restrictions||For Research Use only|
Lund-Johansen, Davis, Bishop et al.: "Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions." in: Cytometry, Vol. 39, Issue 4, pp. 250-9, 2000 (PubMed).
Liu, Tsai, Aird: "Egr-1 gene is induced by the systemic administration of the vascular endothelial growth factor and the epidermal growth factor." in: Blood, Vol. 96, Issue 5, pp. 1772-81, 2000 (PubMed).
Visconti, Gadina, Chiariello et al.: "Importance of the MKK6/p38 pathway for interleukin-12-induced STAT4 serine phosphorylation and transcriptional activity." in: Blood, Vol. 96, Issue 5, pp. 1844-52, 2000 (PubMed).
Lehmann, Janda, Pierreux et al.: "Raf induces TGFbeta production while blocking its apoptotic but not invasive responses: a mechanism leading to increased malignancy in epithelial cells." in: Genes & development, Vol. 14, Issue 20, pp. 2610-22, 2000 (PubMed).
Kim, Ju, Oh et al.: "ERK-1/2 and p38 kinase oppositely regulate nitric oxide-induced apoptosis of chondrocytes in association with p53, caspase-3, and differentiation status." in: The Journal of biological chemistry, Vol. 277, Issue 2, pp. 1332-9, 2002 (PubMed).