Contactin Associated Protein 1 (CNTNAP1) (AA 1-1513) antibody

Antigen: Contactin Associated Protein 1 (CNTNAP1)

Synonyms: P190, CASPR, NRXN4, CNTNAP, NCP1, p190, Caspr, Nrxn4, AI841080, CNTNAP1

Binding Site: AA 1-1513

Clonality: Monoclonal (30)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967781

Additional Information

Alternative name: p190

Immunogen: Human p190

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine), Chicken, Frog

Format: Liquid

Isotype: IgG1

Clone: 30

Description: GTPase activating proteins (GAPs) stimulate the GTP-hydrolyzing activity of GTPases, such as p21[ ras]. Ras-GAP (120kDa) is perhaps the best characterized GAP. p190 is a Ras-GAP associated protein that is tyrosine phosphorylated in transformed and growth factor-stimulated cells. Ras-GAP and p190 are targets of oncoproteins and growth factor receptors. Recombinant p190 enhances GTPase activity of the rho family of GTPases. The complete sequence of p190 exhibits three major regions: an N-terminal region with homology to all GTPases, a middle region homologous to transcriptional repressors, and a C-terminal region the is similar to n-chimerin and other small GTPases.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 190 kDa

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20° C.

Restrictions: For Research Use only

Images

Western blot analysis of p190 on HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of p190.

Human Fibroblast

Publications

Product: Settleman, Narasimhan, Foster et al.: "Molecular cloning of cDNAs encoding the GAP-associated protein p190: implications for a signaling pathway from ras to the nucleus." in: Cell, Vol. 69, Issue 3, pp. 539-49, 1992 (PubMed).

Nakahara, Mueller, Nomizu et al.: "Activation of beta1 integrin signaling stimulates tyrosine phosphorylation of p190RhoGAP and membrane-protrusive activities at invadopodia." in: The Journal of biological chemistry, Vol. 273, Issue 1, pp. 9-12, 1998 (PubMed).

Brandt, Gimona, Hillmann et al.: "Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway." in: The Journal of biological chemistry, Vol. 277, Issue 23, pp. 20903-10, 2002 (PubMed).

Maddox, Burridge: "RhoA is required for cortical retraction and rigidity during mitotic cell rounding." in: The Journal of cell biology, Vol. 160, Issue 2, pp. 255-65, 2003 (PubMed).

Wolf, Draghi, Liang et al.: "p190RhoGAP can act to inhibit PDGF-induced gliomas in mice: a putative tumor suppressor encoded on human chromosome 19q13.3." in: Genes & development, Vol. 17, Issue 4, pp. 476-87, 2003 (PubMed).