Prostaglandin-Endoperoxide Synthase 2 (PTGS2) (AA 368-604) antibody

Antigen: Prostaglandin-Endoperoxide Synthase 2 (PTGS2)

Synonyms: COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, GRIPGHS, Cox-2, Pghs2, TIS10, Cox2, ptgs2, zCOX-2, fj02a10, unp1239, wu:fj02a10, PTGS2, cox2, cox-2, MGC80431, phs-2, hcox-2, pgg/hs, pghs-2, MGC97821, PGHS2, Ptgs2

Binding Site: AA 368-604

Clonality: Monoclonal (33)

Host: Mouse

Reactivity: Rat (Rattus)

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967822

Additional Information

Alternative name: Cox-2

Immunogen: Rat Cox-2

Cross-Reactivity: Mouse (Murine), Human, Chicken

Format: Liquid

Isotype: IgG1

Clone: 33

Description: Cyclooxygenase (Cox) is also known as prostaglandin H synthase or PGH synthase (E.C. 1.14.99.1). It catalyzes the conversion of arachidonate to prostaglandin H2 (PGH2), the precursor of PGE2, PGF2alpha, PGD2, prostacyclin, and thromboxane A2. Cox actually has two different enzymatic activities: a cyclooxygenase that mediates the formation of PGG2 from oxygen and arachidonate and a hydroperoxidase that catalyzes a reduction of PGG2 yielding PGH2. Two Cox genes, Cox-1 and Cox-2, have been isolated in several species. A 4kb mRNA encodes the 604 amino acid Cox-2 protein. The two human Cox isoenzymes are 61% identical in amino acid composition with the active sites being highly conserved. Cox-2 mRNA and protein levels are induced by serum, lipopolysaccharides, growth factors, human chorionic gonadotropin and phorbol testers in various mammalian cell types. It has been shown that interleukin-1alpha (IL-1alpha) induces increased levels of Cox-2 mRNA and protein in human endothelial cells. The sustained increase in Cox-2 is apparently due (at least in part) to IL-1alpha increasing the stability of Cox-2 mRNA. This type of regulatory mechanism may play an important role in chronic inflammatory conditions.
Synonyms: PGHS-2, Cyclooxygenase-2

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.

Molecular Weight: 70 kDa

Comments:

Related Products: ABIN968550, ABIN967389

Synonyms: COX2, COX-2, PHS-2, PGG/HS, PGHS-2, hCox-2, GRIPGHS, Cox-2, Pghs2, TIS10, Cox2, ptgs2, zCOX-2, fj02a10, unp1239, wu:fj02a10, PTGS2, cox2, cox-2, MGC80431, phs-2, hcox-2, pgg/hs, pghs-2, MGC97821, PGHS2, Ptgs2

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Cox-2 on a lysate from mouse macrophages treated with IFNgamma and LPS. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of the Mouse Anti-Cox-2 antibody.

Immunofluoresence staining of mouse macrophages.

Immunohistochemical staining of neurons and endothelial cells from blood vessels (formalin-fixed, citrate buffer pre-treatment, 10X).

Publications

Product: Xie, Cho, Calaycay et al.: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).

Hla, Neilson: "Human cyclooxygenase-2 cDNA." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 89, Issue 16, pp. 7384-8, 1992 (PubMed).

Marrogi, Pass, Khan et al.: "Human mesothelioma samples overexpress both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS2): in vitro antiproliferative effects of a COX-2 inhibitor." in: Cancer research, Vol. 60, Issue 14, pp. 3696-700, 2000 (PubMed).

Giroux, Descoteaux: "Cyclooxygenase-2 expression in macrophages: modulation by protein kinase C-alpha." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 165, Issue 7, pp. 3985-91, 2000 (PubMed).

Shiotani, Denda, Yamamoto et al.: "Increased expression of cyclooxygenase-2 protein in 4-nitroquinoline-1-oxide-induced rat tongue carcinomas and chemopreventive efficacy of a specific inhibitor, nimesulide." in: Cancer research, Vol. 61, Issue 4, pp. 1451-6, 2001 (PubMed).