Cyclin B (AA 14-168) antibody

Antigen: Cyclin B

Binding Site: AA 14-168

Clonality: Monoclonal (18)

Host: Mouse

Reactivity: Human

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Catalog no.: ABIN967833

Additional Information

Immunogen: Human Cyclin B

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 18

Description: The B-type cyclins serve as subunits of the major mitotic protein kinase in many different organisms. In mammals, Cyclin B is a subunit for the cdc2 (Cdk1) protein kinase and is synthesized during late S and G2 phases. Cyclin B associates with inactive cdc2 and facilitates phosphorylation of cdc2 at Thr-14 and Tyr-15. This phosphorylation maintains the inactive state until the end of G2. The inactive Cyclin B-cdc2 complex continues to accumulate in the cytoplasm until the CDC25 phosphatase dephosphorylates Thr-14 and Tyr-15, rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when Cyclin B becomes degraded. This degradation process appears to be ubiquitin-dependent and is necessary for the cell to exit mitosis. Also, the conserved domain in B-type cyclins, known as the P box, is required for CDC25 activation. This antibody is routinely tested by western blot analysis.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 62 kDa

Comments:

Related Products: ABIN968537, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of cyclin B on a Jurkat cell lysate. Lane 1: 1:1000, Lane 2: 1:2000, Lane 3: 1:4000 dilution of the anti- cyclin B antibody.

Immunofluorescence staining of SKN cells (human neuroblastoma).

Publications

Product: Mailand, Falck, Lukas et al.: "Rapid destruction of human Cdc25A in response to DNA damage." in: Science (New York, N.Y.), Vol. 288, Issue 5470, pp. 1425-9, 2000 (PubMed).

Takasaki, Kogure, Takeuchi et al.: "Reactivity of anti-proliferating cell nuclear antigen (PCNA) murine monoclonal antibodies and human autoantibodies to the PCNA multiprotein complexes involved in cell proliferation." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 7, pp. 4780-7, 2001 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).

Miyoshi, Rosner, Nozawa et al.: "Activation of different Wnt/beta-catenin signaling components in mammary epithelium induces transdifferentiation and the formation of pilar tumors." in: Oncogene, Vol. 21, Issue 36, pp. 5548-56, 2002 (PubMed).

Topper, Campbell, Tugendreich et al.: "The dephosphorylated form of the anaphase-promoting complex protein Cdc27/Apc3 concentrates on kinetochores and chromosome arms in mitosis." in: Cell cycle (Georgetown, Tex.), Vol. 1, Issue 4, pp. 282-92, 2002 (PubMed).