rala antibody (AA 35-206)

Catalog No. ABIN967835

The Mouse Monoclonal anti-rala antibody has been validated for WB, IP, IF and IHC (f). It is suitable to detect rala in samples from Rat, Dog and Chicken. There are 4+ publications available.

Quick Overview for rala antibody (AA 35-206) (ABIN967835)

Target: rala (Ras-related Protein Ral-A (rala))

Reactivity: Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This rala antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 8-Ral A

Product Details anti-rala Antibody

Binding Specificity: AA 35-206

Cross-Reactivity: Rat (Rattus), Chicken, Dog (Canine), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Ral A aa. 35-206

Isotype: IgG2a kappa

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-rala antibody (ABIN967835)

Xu, Frankel, Jackson, Rotunda, Boshans, DSouza-Schorey, Foster: "Elevated phospholipase D activity in H-Ras- but not K-Ras-transformed cells by the synergistic action of RalA and ARF6." in: Molecular and cellular biology, Vol. 23, Issue 2, pp. 645-54, (2003) (PubMed).

Kinashi, Katagiri, Watanabe, Vanhaesebroeck, Downward, Takatsu: "Distinct mechanisms of alpha 5beta 1 integrin activation by Ha-Ras and R-Ras." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22590-6, (2000) (PubMed).

Goi, Rusanescu, Urano, Feig: "Ral-specific guanine nucleotide exchange factor activity opposes other Ras effectors in PC12 cells by inhibiting neurite outgrowth." in: Molecular and cellular biology, Vol. 19, Issue 3, pp. 1731-41, (1999) (PubMed).

Olofsson, Chardin, Touchot, Zahraoui, Tavitian: "Expression of the ras-related ralA, rho12 and rab genes in adult mouse tissues." in: Oncogene, Vol. 3, Issue 2, pp. 231-4, (1988) (PubMed).

Target Details for rala

Target: rala (Ras-related Protein Ral-A (rala))

Alternative Name: Ral A

Background: Ral is a low molecular weight GTP-binding protein belonging to the Ras superfamily of GTP-binding proteins and shows 50% amino acid identity to Ras. Ral cDNA clones have been isolated from human placenta, human pheochromocytoma, simian ß-lymphocyte, and marine ray electric lobe cDNA libraries. In humans, cDNA sequences for Ral A and RalB have been determined. The predicted amino acid sequences show 85% identity. Both proteins consist of 206 amino acids with a predicted molecular weight of 24kDa. However, Ral A isolated from human platelets shows an apparent molecular weight of 26-28kDa. Ral A mRNA levels in adult mouse tissues appear to be highest in testes, ovary, and brain, with lower levels found in the liver, spleen, kidney, thymus, heart, and salivary glands. Similar results have been observed in rat tissues. This antibody is routinely tested by western blot analysis.

Molecular Weight: 24-28 kDa

Pathways: Neurotrophin Signaling Pathway, SARS-CoV-2 Protein Interactome