P-Cadherin (AA 72-259) antibody

Antigen: P-Cadherin

Binding Site: AA 72-259

Clonality: Monoclonal (56)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunohistochemistry (IHC)

Catalog no.: ABIN967840

Additional Information

Immunogen: Human P-Cadherin

Cross-Reactivity: Rat (Rattus)

Format: Liquid

Isotype: IgG1

Clone: 56

Description: Cadherins are a family of transmembrane glycoproteins involved in the Ca2+-dependent cell-cell adhesion that occurs in many tissues. Cadherins are similar in their domain structure, Ca2+ and protease sensitivity, and molecular weight. However, these proteins have distinct tissue expression patterns and immunological reactivities. P-Cadherin is localized in placenta while E-Cadherin and N-Cadherin are found in epithelial and neural tissues, respectively. P-Cadherin is an 829 amino acid polypeptide with a putative signal peptide and precursor region, an extracellular domain containing several internal repeats, and a highly hydrophobic transmembrane region. The cytoplasmic domain provides a link to the cytoskeleton through the associated catenin proteins.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 120 kDa

Comments:

Related Products: ABIN968533, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of P-Cadherin on A431 cell lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-P-Cadherin antibody.

Immunofluorescent staining of A431 cells.

Publications

Product: Nose, Nagafuchi, Takeichi: "Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules." in: The EMBO journal, Vol. 6, Issue 12, pp. 3655-61, 1988 (PubMed).

Tao, Edwards, Tubb et al.: "beta-Catenin associates with the actin-bundling protein fascin in a noncadherin complex." in: The Journal of cell biology, Vol. 134, Issue 5, pp. 1271-81, 1996 (PubMed).

Bailey, Biddlestone, Shepherd et al.: "Altered cadherin and catenin complexes in the Barrett's esophagus-dysplasia-adenocarcinoma sequence: correlation with disease progression and dedifferentiation." in: The American journal of pathology, Vol. 152, Issue 1, pp. 135-44, 1998 (PubMed).

Kantak, Kramer: "E-cadherin regulates anchorage-independent growth and survival in oral squamous cell carcinoma cells." in: The Journal of biological chemistry, Vol. 273, Issue 27, pp. 16953-61, 1998 (PubMed).

Holsinger, Ward, Duffield et al.: "The transmembrane receptor protein tyrosine phosphatase DEP1 interacts with p120(ctn)." in: Oncogene, Vol. 21, Issue 46, pp. 7067-76, 2002 (PubMed).