A Kinase (PRKA) Anchor Protein 5 (AKAP5) (AA 180-427) antibody
|Synonyms||H21, AKAP75, AKAP79, P150, Akap79, AKAP150, Gm258, BB098886, 3526401B18Rik, AKAP5, P75, AKAP|
Alternatives Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
|4 references available|
|Quantity||50 µg (250 µg/ml)|
|Price||Product not available in this region.|
|Description||The type II cAMP-dependent Protein Kinase (PKA) is compartmentalized within the cell. To maintain this localization of type II PKAs, the regulatory subunit (RII) interacts with specific RII-anchoring proteins. For instance, attachment of type II PKA to the cytoskeleton occurs through the binding of RII to microtubule-associated protein 2 (MAP2). In brain, several proteins have been identified as PKA type II anchoring proteins and form a family named AKAP (A-Kinase Anchor Proteins). AKAP79 is a 79kDa human RII-anchoring protein. AKAP, PKA type II, and calcineurin (PP2B) can form a tertiary complex, suggesting that both PKA and calcineurin are targeted by a common protein to subcellular sites where they regulate the phosphorylation status of key substrates.|
1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
|Molecular Weight||79 kDa|
Related Products: ABIN968552, ABIN967389
|Purification||Purified from tissue culture supernatant or ascites by affinity chromatography.|
|Buffer||Aqueous buffered solution containing BSA, glycerol.|
|Preservative||0.09% Sodium azide.|
|Storage||Store undiluted at -20°C.|
|Restrictions||For Research Use only|
|Western blot analysis of AKAP79 on SW13 lysate. Lane 1: 1:250, lane 2: 1:500, lane 3: 1:1000 dilution of anti-AKAP79. Immunofluorescent staining of endothelial cells. anti-A Kinase (PRKA) Anchor Protein 5 (AKAP5) (AA 180-427) antibody (Image 3)|
Carr, Stofko-Hahn, Fraser et al.: "Localization of the cAMP-dependent protein kinase to the postsynaptic densities by A-kinase anchoring proteins. Characterization of AKAP 79." in: The Journal of biological chemistry, Vol. 267, Issue 24, pp. 16816-23, 1992 (PubMed).
Coghlan, Perrino, Howard et al.: "Association of protein kinase A and protein phosphatase 2B with a common anchoring protein." in: Science (New York, N.Y.), Vol. 267, Issue 5194, pp. 108-11, 1995 (PubMed).
Jicha, Weaver, Lane et al.: "cAMP-dependent protein kinase phosphorylations on tau in Alzheimer's disease." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 19, Issue 17, pp. 7486-94, 1999 (PubMed).
Schillace, Andrews, Liberty et al.: "Identification and characterization of myeloid translocation gene 16b as a novel a kinase anchoring protein in T lymphocytes." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 168, Issue 4, pp. 1590-9, 2002 (PubMed).
|Hosts||Rabbit (25), Mouse (1)|
|Reactivities||Human (24), Rat (Rattus) (21), Cow (Bovine) (20), Dog (Canine) (20), Mouse (Murine) (20), Horse (Equine) (18), Pig (Porcine) (18), Rabbit (18)|
|Applications||Immunofluorescence (IF) (17), Western Blotting (WB) (11), ELISA (7), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (3), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (3), Immunoelectron Microscopy (IEM) (1)|
|Conjugates||Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Biotin (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), HRP (1), PE (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||AA 428-449 (1), Internal Region (1), N-Term (1)|