AKAP5 antibody (AA 1-80)

Catalog No. ABIN7213526

This Rabbit Polyclonal antibody specifically detects AKAP5 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for AKAP5 antibody (AA 1-80) (ABIN7213526)

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-AKAP5 Antibody

Binding Specificity: AA 1-80

Purpose: Rabbit Anti-AKAP 79 Polyclonal Antibody

Specificity: AKAP 79 Polyclonal Antibody detects endogenous levels of AKAP 79 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the N-terminal region of human AKAP 79 at AA range: 1-80

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AKAP5

Target: AKAP5 (A Kinase (PRKA) Anchor Protein 5 (AKAP5))

Alternative Name: AKAP 79

Background: AKAP5, AKAP79, A-kinase anchor protein 5, AKAP-5, A-kinase anchor protein 79 kDa, AKAP 79, H21, cAMP-dependent protein kinase regulatory subunit II high affinity-binding proteinThe A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. AKAP5 encodes a member of the AKAP family. The encoded protein (A-kinase anchoring protein 5) binds to the RII-beta regulatory subunit of PKA, and also to protein kinase C and the phosphatase calcineurin. It is predominantly expressed in cerebral cortex and may anchor the PKA protein at postsynaptic densities (PSD) and be involved in the regulation of postsynaptic events. It is also expressed in T lymphocytes and may function to inhibit interleukin-2 transcription by disrupting calcineurin-dependent dephosphorylation of NFAT.

Molecular Weight: 47kD

Gene ID: 9495

UniProt: P24588

Pathways: cAMP Metabolic Process