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NOS2 antibody (Nitric Oxide Synthase 2, Inducible) (AA 961-1144)

Details for Product anti-NOS2 Antibody No. ABIN967905, Supplier: Log in to see
Antigen
  • HEP-NOS
  • i-NOS
  • INO1
  • INOS
  • iNOS
  • iNos
  • IPS
  • IPS-1
  • IPS 1
  • NOS
  • Nos-2
  • NOS-II
  • NOS2
  • NOS2a
  • NOS2A
  • Nos2a
Alternatives
anti-Mouse (Murine) NOS2 antibody for Immunocytochemistry
Epitope
AA 961-1144
53
28
15
13
13
13
11
10
9
7
7
7
6
5
5
5
5
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
231
139
73
13
6
3
2
2
2
2
2
Host
Mouse
252
50
6
Clonality (Clone)
Monoclonal ()
Conjugate
This NOS2 antibody is un-conjugated
12
11
5
5
5
4
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
220
136
84
73
63
43
36
25
19
18
11
6
3
2
1
1
1
1
1
Supplier
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Immunogen Mouse iNOS aa. 961-1144
Clone 6-iNOS-NOS Type II
Isotype IgG2a
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Please refer to us for technical protocols.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name iNOS/NOS Type II (NOS2 Antibody Abstract)
Background Nitric oxide synthase (NOS), a cell-type specific enzyme, catalyzes the synthesis of nitric oxide (NO). NO is a short-lived radical that transmits cellular signals involved in vasorelaxation, neurotransmission, and cytotoxicity. In macrophages and other cell types, NOS (iNOS or macNOS) activity increases following exposure to cytokines (IFN-gamma, TNF-alpha, and IL-1) and microbial products (lipopolysaccharide (LPS)), iNOS is activated independently of Ca2+/calmodulin and its level of expression is tightly controlled by several transcription factors, including NFkappaB. Data indicates that TGF-beta affects translation of iNOS mRNA and decreases iNOS protein stability. Normally undetectable in brain tissue, iNOS mRNA has been observed in CNS tissues of animals under experimental pathologic conditions. iNOS and nNOS share 51% amino acid homology with the greatest degree of divergence in the calmodulin binding domain.
Molecular Weight 130 kDa
Pathways Retinoic Acid Receptor Signaling Pathway
Comment

Related Products: ABIN968550, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-NOS2 antibody (Nitric Oxide Synthase 2, Inducible) (AA 961-1144) (ABIN967905) Western blot analysis of iNOS/NOS Type II on a cell lysate from mouse macrophages (RA...
Immunofluorescence (IF) image for anti-NOS2 antibody (Nitric Oxide Synthase 2, Inducible) (AA 961-1144) (ABIN967905) Immunofluorescence staining of mouse macrophages stimulated with 10 ng/mL IFNgamma an...
Product cited in: Heneka, Klockgether, Feinstein: "Peroxisome proliferator-activated receptor-gamma ligands reduce neuronal inducible nitric oxide synthase expression and cell death in vivo." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 20, Issue 18, pp. 6862-7, 2000 (PubMed).

Marrogi, Pass, Khan, Metheny-Barlow, Harris, Gerwin: "Human mesothelioma samples overexpress both cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (NOS2): in vitro antiproliferative effects of a COX-2 inhibitor." in: Cancer research, Vol. 60, Issue 14, pp. 3696-700, 2000 (PubMed).

Miles, Bowman, Rao, Baatz, Huffman: "Pulmonary surfactant inhibits LPS-induced nitric oxide production by alveolar macrophages." in: The American journal of physiology, Vol. 276, Issue 1 Pt 1, pp. L186-96, 1999 (PubMed).

Sciorati, Rovere, Ferrarini, Heltai, Manfredi, Clementi: "Autocrine nitric oxide modulates CD95-induced apoptosis in gammadelta T lymphocytes." in: The Journal of biological chemistry, Vol. 272, Issue 37, pp. 23211-5, 1997 (PubMed).

Xie, Cho, Calaycay, Mumford, Swiderek, Lee, Ding, Troso, Nathan: "Cloning and characterization of inducible nitric oxide synthase from mouse macrophages." in: Science (New York, N.Y.), Vol. 256, Issue 5054, pp. 225-8, 1992 (PubMed).