Adaptin gamma (AA 642-821) antibody

Antigen: Adaptin gamma

Binding Site: AA 642-821

Clonality: Monoclonal (88)

Host: Mouse

Reactivity: Mouse (Murine)

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Catalog no.: ABIN967934

Additional Information

Immunogen: Mouse Adaptin gamma

Cross-Reactivity: Rat (Rattus), Human, Dog (Canine)

Format: Liquid

Isotype: IgG1

Clone: 88

Description: Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain ( sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. Adaptin gamma shows more homology with Adaptin alpha than with Adaptin beta. The conserved regions between Adaptins gamma and alpha could be important for binding to other components of the AP-1 and AP-2 complexes, respectively.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Molecular Weight: 104 kDa

Comments:

Related Products: ABIN968539, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Restrictions: For Research Use only

Images

Western blot analysis of Adaptin gamma on PC12 cell lysate. Lane 1: 1:5000, lane 2: 1:10000, lane 3: 1:20000 dilution of anti-Adaptin gamma.

Immunofluorescent staining of Rat Neurons with anti-Adaptin gamma antibody.

Publications

Product: Robinson: "Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2319-26, 1991 (PubMed).

Wasiak, Legendre-Guillemin, Puertollano et al.: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, 2002 (PubMed).

Eskelinen, Illert, Tanaka et al.: "Role of LAMP-2 in lysosome biogenesis and autophagy." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3355-68, 2002 (PubMed).

Kalthoff, Groos, Kohl et al.: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, 2002 (PubMed).

Nagahama, Suzuki, Hamada et al.: "SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 262-73, 2003 (PubMed).