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gamma 1 Adaptin antibody (Adaptor-Related Protein Complex 1, gamma 1 Subunit) (AA 642-821)

Details for Product anti-AP1G1 Antibody No. ABIN967935, Supplier: Log in to see
Antigen
  • wu:fc30a11
  • AP1G1
  • ap1g1
  • AA409002
  • AU041323
  • AW551707
  • Adtg
  • D8Ertd374e
  • ADTG
  • CLAPG1
  • adtg
  • zgc:56079
Epitope
AA 642-821
13
7
3
2
2
2
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
60
44
42
5
3
2
1
1
Host
Mouse
47
11
8
Clonality (Clone)
Monoclonal ()
Conjugate
This gamma 1 Adaptin antibody is un-conjugated
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
39
22
18
13
10
7
4
2
Supplier
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Immunogen Mouse Adaptin gamma aa. 642-821
Clone 88-Adaptin gamma
Isotype IgG1
Cross-Reactivity Rat (Rattus), Human, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name Adaptin gamma (AP1G1 Antibody Abstract)
Background Sorting of integral membrane proteins at various stages of the endocytic and secretory pathways is mediated by vesicular trafficking between a variety of organelles. Two sorting signals are tyrosine-based and dileucine-based signals that interact with heterotetrameric adaptor protein complexes (AP-1, AP-2, AP-3, and AP-4), which are associated with the vesicle coats. These coatomers contain two large Adaptin proteins (gamma, alpha, delta, or epsilon and beta1, beta2, beta3, or beta4, respectively) that are noncovalently linked to one medium chain (µ1, µ2, µ3, or µ4) and one small chain ( sigma1, sigma2, sigma3, or sigma4). The AP-1 and AP-3 complexes are involved in protein sorting from the TGN and endosomes, while AP-2 adaptor complexes are involved in clathrin-mediated endocytosis. Adaptin gamma shows more homology with Adaptin alpha than with Adaptin beta. The conserved regions between Adaptins gamma and alpha could be important for binding to other components of the AP-1 and AP-2 complexes, respectively.
Molecular Weight 104 kDa
Pathways
Comment

Related Products: ABIN968539, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 μg/mL
Buffer Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store undiluted at -20°C.
Supplier Images
Western Blotting (WB) image for anti-gamma 1 Adaptin antibody (Adaptor-Related Protein Complex 1, gamma 1 Subunit) (AA 642-821) (ABIN967935) Western blot analysis of Adaptin gamma on PC12 cell lysate. Lane 1: 1:5000, lane 2: 1...
Immunofluorescence (IF) image for anti-gamma 1 Adaptin antibody (Adaptor-Related Protein Complex 1, gamma 1 Subunit) (AA 642-821) (ABIN967935) Immunofluorescent staining of Rat Neurons with anti-Adaptin gamma antibody.
 image for anti-gamma 1 Adaptin antibody (Adaptor-Related Protein Complex 1, gamma 1 Subunit) (AA 642-821) (ABIN967935) anti-Adaptor-Related Protein Complex 1, gamma 1 Subunit (AP1G1) (AA 642-821) antibody (Image 3)
Product cited in: Nagahama, Suzuki, Hamada et al.: "SVIP is a novel VCP/p97-interacting protein whose expression causes cell vacuolation." in: Molecular biology of the cell, Vol. 14, Issue 1, pp. 262-73, 2003 (PubMed).

Kalthoff, Groos, Kohl et al.: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, 2002 (PubMed).

Eskelinen, Illert, Tanaka et al.: "Role of LAMP-2 in lysosome biogenesis and autophagy." in: Molecular biology of the cell, Vol. 13, Issue 9, pp. 3355-68, 2002 (PubMed).

Wasiak, Legendre-Guillemin, Puertollano et al.: "Enthoprotin: a novel clathrin-associated protein identified through subcellular proteomics." in: The Journal of cell biology, Vol. 158, Issue 5, pp. 855-62, 2002 (PubMed).

Robinson: "Cloning and expression of gamma-adaptin, a component of clathrin-coated vesicles associated with the Golgi apparatus." in: The Journal of cell biology, Vol. 111, Issue 6 Pt 1, pp. 2319-26, 1991 (PubMed).