Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27) (AA 145-343) antibody

Antigen: Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27)

Synonyms: APC3, HNUC, CDC27Hs, D0S1430E, D17S978E, wu:fb54g06, wu:fc88c12, AI452358, BC023187, MGC80529, CDC27, MGC145551, NV10210

Binding Site: AA 145-343

Clonality: Monoclonal (35)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)

Catalog no.: ABIN967981

Additional Information

Alternative name: Cdc27

Immunogen: Human CDC27

Cross-Reactivity: Dog (Canine), Rat (Rattus), Mouse (Murine)

Format: Liquid

Isotype: IgG2b

Clone: 35

Description: CDC16 and CDC27 were discovered in yeast as genes essential for the G2/M transition of the cell cycle. The human homologues of CDC16 and CDC27 were cloned and found to encode proteins of 72kDa and 97kDa, respectively. Biochemical and subcellular localization experiments have shown that CDC16 and CDC27 are novel components of the mitotic spindle centrosome in human cells. In HeLa cells, there are about 100,000 copies of each protein per cell. Although its precise role during mitosis remains to be determined, CDC16 appears to be required for cyclin degradation in yeast. Wild type CDC27 is required for the initiation of anaphase. Immunofluorescence studies have shown that both proteins are components of the centrosome in the absence of spindle fibers.

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Molecular Weight: 97 kDa

Comments:

Related Products: ABIN968535, ABIN967389

Application Details

Concentration: 250 µg/ml

Purity: Purified

Purification: Purified from tissue culture supernatant or ascites by affinity chromatography.

Buffer: Aqueous buffered solution containing BSA, glycerol.

Preservative: 0.09% Sodium azide.

Storage: Store undiluted at -20°C.

Research Area: Proteolysis / Ubiquitin, Cell Cycle

Restrictions: For Research Use only

Images

Western blot analysis of CDC27 on HeLa lysate. Lane 1: 1:500, lane 2: 1:1000, lane 3: 1:2000 dilution of anti-CDC27.

Immunofluorescent staining of HeLa cells.

Publications

Product: Tugendreich, Tomkiel, Earnshaw et al.: "CDC27Hs colocalizes with CDC16Hs to the centrosome and mitotic spindle and is essential for the metaphase to anaphase transition." in: Cell, Vol. 81, Issue 2, pp. 261-8, 1995 (PubMed).

Tugendreich, Boguski, Seldin et al.: "Linking yeast genetics to mammalian genomes: identification and mapping of the human homolog of CDC27 via the expressed sequence tag (EST) data base." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 21, pp. 10031-5, 1993 (PubMed).

Kramer, Scheuringer, Podtelejnikov et al.: "Mitotic regulation of the APC activator proteins CDC20 and CDH1." in: Molecular biology of the cell, Vol. 11, Issue 5, pp. 1555-69, 2000 (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, 2002 (PubMed).