EEA1 antibody (AA 3-281)

Catalog No. ABIN967983

This anti-EEA1 antibody is a Mouse Monoclonal antibody detecting EEA1 in WB, IHC, IP and IF. Suitable for Human, Rat, Dog and Chicken. This Primary Antibody has been cited in 5+ publications.

Quick Overview for EEA1 antibody (AA 3-281) (ABIN967983)

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Reactivity: Human, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This EEA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 14-EEA1

Product Details anti-EEA1 Antibody

Binding Specificity: AA 3-281

Cross-Reactivity: Rat (Rattus), Chicken, Dog (Canine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human EEA1 aa. 3-281

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-EEA1 antibody (ABIN967983)

van Dam, Stoorvogel: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, (2002) (PubMed).

Yan, Han, Miao, Greengard, Xu: "The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36788-96, (2001) (PubMed).

Allan, Moyer, Balch: "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion." in: Science (New York, N.Y.), Vol. 289, Issue 5478, pp. 444-8, (2000) (PubMed).

Mullock, Smith, Ihrke, Bright, Lindsay, Parkinson, Brooks, Parton, James, Luzio, Piper: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, (2000) (PubMed).

Mu, Callaghan, Steele-Mortimer, Stenmark, Parton, Campbell, McCluskey, Yeo, Tock, Toh: "EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13503-11, (1995) (PubMed).

Target Details for EEA1

Target: EEA1 (Early Endosome Antigen 1 (EEA1))

Alternative Name: EEA1

Background: Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly alpha-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine finger motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity. This antibody is routinely tested by western blot analysis.

Molecular Weight: 180 kDa

Pathways: Toll-Like Receptors Cascades