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Early Endosome Antigen 1 (EEA1) (AA 3-281) antibody

Details for Product No. ABIN967983
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Antigen
Synonyms LOC562732, MST105, MSTP105, ZFYVE2, A430109M19Rik, B230358H09Rik, im:7151048, si:ch211-276p10.2
Epitope
AA 3-281
(9), (5), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human
(50), (28), (21), (14), (13), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Host
Mouse
(39), (7), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunofluorescence (IF)
(38), (10), (8), (7), (7), (7), (6), (4), (2)
Pubmed 5 references available
Quantity 150 µg
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Catalog No. ABIN967983
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Immunogen Human EEA1
Clone Ghr2
Isotype IgG1
Cross-Reactivity Rat (Rattus), Chicken, Dog (Canine)
Characteristics 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
Purification Purified from tissue culture supernatant or ascites by affinity chromatography.
Alternative Name EEA1
Background Early endosomes are a major cytoplasmic sorting compartment from which receptors and ligands may be distributed to various sites within the cell. Early endosome antigen 1 (EEA1) is a 180 kDa hydrophilic peripheral membrane protein present in cytosol and membrane fractions. Immunofluorescence studies show that EEA1 colocalizes to early endosomes with transferrin receptor and Rab5, but not with the late endosome-localizing Rab7. EEA1 is predominantly alpha-helical and shares 17-20% sequence identity with the myosins. It contains a calmodulin-binding IQ motif and metal-binding cysteine finger motifs. It is thought that EEA1 is required for vesicular transport of proteins through early endosomes and that these finger motifs are required for this activity. This antibody is routinely tested by western blot analysis.
Molecular Weight 180 kDa
Research Area Neurology, Organelles
Comment

Related Products: ABIN968545, ABIN967389

Restrictions For Research Use only
Format Liquid
Concentration 250 µg/ml
Buffer Aqueous buffered solution containing BSA, glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Supplier Images
anti-Early Endosome Antigen 1 (EEA1) (AA 3-281) antibody Western blot analysis of EEA1 on rat brain lysate. Lane 1: 1:2500, lane 2: 1:5000, lane 3: 1:10000 dilution of anti-EEA1 antibody.
anti-Early Endosome Antigen 1 (EEA1) (AA 3-281) antibody (2) Immunostaining on Human Smooth Muscle
anti-Early Endosome Antigen 1 (EEA1) (AA 3-281) antibody (3) anti-Early Endosome Antigen 1 (EEA1) (AA 3-281) antibody (Image 3)
Product cited in: Mu, Callaghan, Steele-Mortimer et al.: "EEA1, an early endosome-associated protein. EEA1 is a conserved alpha-helical peripheral membrane protein flanked by cysteine "fingers" and contains a calmodulin-binding IQ motif." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13503-11, 1995 (PubMed).

Allan, Moyer, Balch: "Rab1 recruitment of p115 into a cis-SNARE complex: programming budding COPII vesicles for fusion." in: Science (New York, N.Y.), Vol. 289, Issue 5478, pp. 444-8, 2000 (PubMed).

Mullock, Smith, Ihrke et al.: "Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and Is required for late endosome-lysosome fusion." in: Molecular biology of the cell, Vol. 11, Issue 9, pp. 3137-53, 2000 (PubMed).

Yan, Han, Miao et al.: "The transmembrane domain of the Alzheimer's beta-secretase (BACE1) determines its late Golgi localization and access to beta -amyloid precursor protein (APP) substrate." in: The Journal of biological chemistry, Vol. 276, Issue 39, pp. 36788-96, 2001 (PubMed).

van Dam, Stoorvogel: "Dynamin-dependent transferrin receptor recycling by endosome-derived clathrin-coated vesicles." in: Molecular biology of the cell, Vol. 13, Issue 1, pp. 169-82, 2002 (PubMed).

Validation Images
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